验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, NIH/3T3 cells, human cerebellum tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | human kidney tissue, human heart tissue, human lung tissue, human ovary tissue, human placenta tissue, human skin tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 12 publications below |
产品信息
16094-1-AP targets KPTN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag8785 Product name: Recombinant human KPTN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 79-436 aa of BC009249 Sequence: EIVSIDTFNKSPPKRGLVVGITFIKDSGDKGSPFLNIYCDYEPGSEYNLDSIAQSCLNLELQFTPFQLCHAEVQVGDQLETVFLLSGNDPAIHLYKENEGLHQFEEQPVENLFPELTNLTSSVLWLDVHNFPGTSRRLSALGCQSGYVRVAHVDQRSREVLQMWSVLQDGPISRVIVFSLSAAKETKDRPLQDEYSVLVASMLEPAVVYRDLLNRGLEDQLLLPGSDQFDSVLCSLVTDVDLDGRPEVLVATYGQELLCYKYRGPESGLPEAQHGFHLLWQRSFSSPLLAMAHVDLTGDGLQELAVVSLKGVHILQHSLIQASELVLTRLRHQVEQRRRRLQGLEDGAGAGPAENAAS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kaptin (actin binding protein) |
| 别名 | KICSTOR complex protein kaptin, Kaptin, Actin-associated protein 2E4 |
| 计算分子量 | 436 aa, 48 kDa |
| 观测分子量 | 48 kDa |
| GenBank蛋白编号 | BC009249 |
| 基因名称 | KPTN |
| Gene ID (NCBI) | 11133 |
| RRID | AB_2134007 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y664 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Kaptin, also named as KPTN and Actin-associated protein 2E4, originally isolated from human blood platelets and likely to be involved in the actin rearrangements occurring during activation. It plays a unique role in the actin rearrangements that accompany platelet activation and stereocilia formation. Kaptin is necessary for normal neuromorphogenesis. It may play a role in producing the sensory apparatus in hair cells.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for KPTN antibody 16094-1-AP | Download protocol |
| IHC protocol for KPTN antibody 16094-1-AP | Download protocol |
| WB protocol for KPTN antibody 16094-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nature KICSTOR recruits GATOR1 to the lysosome and is necessary for nutrients to regulate mTORC1.
| ||
Proc Natl Acad Sci U S A Rag-Ragulator is the central organizer of the physical architecture of the mTORC1 nutrient-sensing pathway | ||
Cell Rep CDK5-PRMT1-WDR24 signaling cascade promotes mTORC1 signaling and tumor growth
| ||
