验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells |
| Positive IP detected in | Jurkat cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
| CoIP | See 1 publications below |
产品信息
13963-1-AP targets KPNA5 in WB, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 文献引用应用 | WB, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, rat |
| 免疫原 |
CatNo: Ag4920 Product name: Recombinant human KPNA5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-231 aa of BC047409 Sequence: MDAMASPGKDNYRMKSYKNKALNPQEMRRRREEEGIQLRKQKREEQLFKRRNVYLPRNDESMLESPIQDPDISSTVPIPEEEVVTTDMVQMIFSNNADQQLTATQKFRKLLSKEPNPPIDQVIQKPGVVQRFVKFLERNENCTLQFEAAWALTNIASGTFLHTKVVIETGAVPIFIKLLNSEHEDVQEQAVWALGNIAGDNAECRDFVLNCEILPPLLELLTNSNRLTTTR 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | karyopherin alpha 5 (importin alpha 6) |
| 别名 | Importin subunit alpha 6, IPOA6, Karyopherin subunit alpha 5, KPNA5, SRP6 |
| 计算分子量 | 60 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC047409 |
| 基因名称 | KPNA5 |
| Gene ID (NCBI) | 3841 |
| RRID | AB_2133847 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15131 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for KPNA5 antibody 13963-1-AP | Download protocol |
| WB protocol for KPNA5 antibody 13963-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Diabetologia Saturated fatty acids entrap PDX1 in stress granules and impede islet beta cell function. | ||
Virol Sin African Swine Fever Virus MGF360-12L Inhibits Type I Interferon Production by Blocking the Interaction of Importin α and NF-κB Signaling Pathway. | ||
J Immunol Cutting edge: tubulin α functions as an adaptor in NFAT-importin β interaction. | ||
PLoS One An interaction network predicted from public data as a discovery tool: application to the Hsp90 molecular chaperone machine. | ||
J Neurosci miR-153 prevents NRF2 nuclear translocation to drive hypoperfusion-related cognitive deficits by targeting KPNA5 |
