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验证数据展示

  • WB analysis using 82898-1-RR (same clone as 82898-1-PBS)

    WB analysis using 82898-1-RR (same clone as 82898-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82898-1-RR (KPNA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82898-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 82898-1-RR (same clone as 82898-1-PBS)

    IF Staining of U2OS using 82898-1-RR (same clone as 82898-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KPNA4 antibody (82898-1-RR, Clone: 230098H3 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 82898-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 82898-1-RR (same clone as 82898-1-PBS)

    FC experiment of HeLa using 82898-1-RR (same clone as 82898-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.13 ug Anti-Human KPNA4 (82898-1-RR, Clone:230098H3) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:166 (red), or 0.13 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82898-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 82898-1-RR (same clone as 82898-1-PBS)

    WB analysis of A549 using 82898-1-RR (same clone as 82898-1-PBS)

    WB result of KPNA4 antibody (82898-1-RR; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KPNA4 transfected A549 cells. This data was developed using the same antibody clone with 82898-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82898-1-RR

    Affinity and Kinetic Characterization of 82898-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82898-1-RR against Human KPNA4 were performed. The affinity constant is 0.417 nM.

  • Featured Product
  • KD/KO验证
    • {{ptg:ProductFeaturesInfo}}

KPNA4 Recombinant monoclonal antibody, PBS Only

KPNA4 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 82898-1-PBS

产品说明书

CloneNo. 230098H3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

QIP1, KPNA 4, Karyopherin subunit alpha-4, IPOA3, Importin subunit alpha-3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

82898-1-PBS targets KPNA4 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3133

Product name: Recombinant human KPNA4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC034493

Sequence: MADNEKLDNQRLKNFKNKGRDLETMRRQRNEVVVELRKNKRDEHLLKRRNVPHEDICEDSDIDGDYRVQNTSLEAIVQNASSDNQGIQLSAVQAARKLLSSDRNPPIDDLIKSGILPILVHCLERDDNPSLQFEAAWALTNIASGTSEQTQAVVQSNAVPLFLRLLHSPHQNVCEQAVWALGNIIGDGPQCRDYVISLGVVKPLLSFISPSIPITFLRNVTWVMVNLCRHKDPPPPMETIQEILPALCVLIHHTDVNILVDTVWALSYLTDAGNEQIQMVIDSGIVPHLVPLLSHQEVKVQTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHPKEKINKEAVWFL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 karyopherin alpha 4 (importin alpha 3)
别名 QIP1, KPNA 4, Karyopherin subunit alpha-4, IPOA3, Importin subunit alpha-3
计算分子量 521 aa, 58 kDa
观测分子量 58 kDa
GenBank蛋白编号BC034493
基因名称 KPNA4
Gene ID (NCBI) 3840
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDO00629
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C