验证数据展示
经过测试的应用
| Positive IHC detected in | mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
21532-1-AP targets KNCN in IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag16160 Product name: Recombinant human KNCN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2-75 aa of BC101295 Sequence: GLLILAYPFLKARFNLDHILPTIGSLRIHPHPGADHGEGRSSTNGNKEGARSSLSTVSRTLEKLKPGTRGAEEC 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kinocilin |
| 别名 | Kino, kinocilin |
| 计算分子量 | 124 aa, 13 kDa |
| GenBank蛋白编号 | BC101295 |
| 基因名称 | KNCN |
| Gene ID (NCBI) | 148930 |
| RRID | AB_3742043 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | A6PVL3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Kinocilin (KNCN) was first detected in the kinocilia of vestibular and auditory hair cells at embryonic days 14.5 and 18.5, respectively. In mature auditory hair cells, KNCN was present at the cuticular plate, at the base of each stereocilium. KNCN was also expressed by the pillar cells and Deiters cells, both of which contain prominent transcellular and apical bundles of microtubules. KNCN expression was detected in both cochlear and vestibular hair cells. KNCN plays a role in stabilizing dense microtubular networks or invesicular trafficking (PMID: 30254566).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for KNCN antibody 21532-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
