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CoraLite® Plus 647 Anti-Mouse KLRG1 (2F1)

KLRG1 Monoclonal Antibody for FC

Host / Isotype

Golden Syrian Hamster / IgG

Reactivity

Mouse

Applications

FC

Conjugate

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

2F1

Cat No : CL647-65084

Print datasheet

Synonyms

2F1 Ag, Klrg1, MAFA, MAFA L

验证数据展示

  • FC experiment of mouse splenocytes using CL647-65084

    FC experiment of mouse splenocytes using CL647-65084

    1x10^6 mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse KLRG1 (2F1) (CL647-65084, Clone: 2F1) or 0.25 ug Isotype Control, and 0.25 ug FITC Anti-Mouse NK1.1 (CD161) (PK136) (FITC-65138, Clone: PK136). Cells were not fixed.

经过测试的应用

Positive FC detected inmouse splenocytes
For other applications, we recommend the unconjugated version of this antibody, 65084-1-Ig

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-65084 targets KLRG1 in FC applications and shows reactivity with Mouse samples.

Tested Applications FC
Tested Reactivity Mouse
Immunogen N/A 种属同源性预测
Host / Isotype Golden Syrian Hamster / IgG
Class Monoclonal
Type Antibody
Full Name killer cell lectin-like receptor subfamily G, member 1
Synonyms 2F1 Ag, Klrg1, MAFA, MAFA L
GenBank Accession NumberBC101953
Gene Symbol Klrg1
Gene ID (NCBI) 50928
RRIDAB_2920251
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAffinity purification
UNIPROT IDO88713
Storage Buffer PBS with 0.1% sodium azide and 0.5% BSA, pH 7.3.
Storage ConditionsStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

背景介绍

KLRG1 (killer cell lectin-like receptor subfamily G, member 1) is a C-type lectin inhibitory receptor that contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) in its cytoplasmic domain. KLRG1 is expressed by subsets of NK and T cells, existing both as a monomer and as a disulfide-linked homodimer (PMID: 10741410; 19604491). It is considered to be a cell differentiation marker for NK and T cells and is strongly induced by viral and other infections (PMID: 20373518). Through interactions with members of the cadherin family, KLRG1 plays an inhibitory role on NK- and T-cell function (PMID: 19009530; 20373518).

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 KLRG1 antibody CL647-65084Download protocol
Standard Protocols
Click here to view our Standard Protocols