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验证数据展示

  • WB analysis using 31532-1-AP

    WB analysis using 31532-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31532-1-AP (KLRAQ1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 31532-1-AP

    IHC staining of human stomach cancer using 31532-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 31532-1-AP (PPP1R21 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse cerebellum using 31532-1-AP

    IF Staining of mouse cerebellum using 31532-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using KLRAQ1 antibody (31532-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PPP1R21 Polyclonal antibody

PPP1R21 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 31532-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, ELISA

别名

KLRAQ1, NEDHFBA, KLRAQ motif containing 1, FERRY2, CCDC128

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, THP-1 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse cerebellum tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31532-1-AP targets PPP1R21 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 PPP1R21 fusion protein Ag35449 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KLRAQ motif containing 1
别名 KLRAQ1, NEDHFBA, KLRAQ motif containing 1, FERRY2, CCDC128
观测分子量88 kDa
GenBank蛋白编号BC111059
基因名称 KLRAQ1
Gene ID (NCBI) 129285
RRIDAB_3670023
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ6ZMI0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPP1R21, also known as KLRAQ1, CCDC12 and FERRY2, encodes a conserved protein involved in endosomal maturation. It has many isoforms and may play a role in the endosomal sorting process or in the endosome maturation pathway.

实验方案

Product Specific Protocols
WB protocol for PPP1R21 antibody 31532-1-APDownload protocol
IHC protocol for PPP1R21 antibody 31532-1-APDownload protocol
IF protocol for PPP1R21 antibody 31532-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols