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验证数据展示

  • WB analysis using 12264-1-AP

    WB analysis using 12264-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12264-1-AP (Kallikrein 6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 12264-1-AP

    IHC staining of human breast cancer using 12264-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12264-1-AP (Kallikrein 6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 12264-1-AP

    IHC staining of human breast cancer using 12264-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12264-1-AP (Kallikrein 6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Kallikrein 6 Polyclonal antibody

Kallikrein 6 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 12264-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

KLK6, EC:3.4.21.-, Kallikrein-6, PRSS18, PRSS9

缓冲液配方:  PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMDA-MB-468 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12264-1-AP targets Kallikrein 6 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag2930

Product name: Recombinant human KLK6 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-244 aa of BC015525

Sequence: MKKLMVVLSLIAAAWAEEQNKLVHGGPCDKTSHPYQAALYTSGHLLCGGVLIHPLWVLTAAHCKKPNLQVFLGKHNLRQRESSQEQSSVVRAVIHPDYDAASHDQDIMLLRLARPAKLSELIQPLPLERDCSANTTSCHILGWGKTADGDFPDTIQCAYIHLVSREECEHAYPGQITQNMLCAGDEKYGKDSCQGDSGGPLVCGDHLRGLVSWGNIPCGSKEKPGVYTNVCRYTNWIQKTIQAK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kallikrein-related peptidase 6
别名 KLK6, EC:3.4.21.-, Kallikrein-6, PRSS18, PRSS9
计算分子量 244 aa, 28 kDa
GenBank蛋白编号BC015525
基因名称 KLK6
Gene ID (NCBI) 5653
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ92876
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kallikrein 6 (KLK6, also named neurosin) is a secreted serine protease encoded by the KLK6 gene on human chromosome 19q13.41. It is highly expressed in the central nervous system and epithelial tissues. KLK6 participates in extracellular matrix remodeling, regulates amyloid and α-synuclein metabolism, and modulates inflammatory responses. Dysregulated KLK6 facilitates tumor invasion and metastasis in multiple malignancies. It is regarded as a valuable diagnostic and prognostic biomarker for cancers and neurodegenerative diseases, representing a promising therapeutic target. (PMID: 35883559)

实验方案

Product Specific Protocols
IHC protocol for Kallikrein 6 antibody 12264-1-APDownload protocol
WB protocol for Kallikrein 6 antibody 12264-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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