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验证数据展示

  • IHC staining of human liver cancer using 27515-1-AP

    IHC staining of human liver cancer using 27515-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27515-1-AP (KLK15 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human liver cancer using 27515-1-AP

    IHC staining of human liver cancer using 27515-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27515-1-AP (KLK15 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of SMMC-7721 using 27515-1-AP

    WB analysis of SMMC-7721 using 27515-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 27515-1-AP (KLK15 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

KLK15 Polyclonal antibody

KLK15 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27515-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, IHC, ELISA

别名

ACO, ACO protease, HSRNASPH, Kallikrein 15, KLK15

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inSMMC-7721 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27515-1-AP targets KLK15 in WB, IHC, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag26232

Product name: Recombinant human KLK15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 109-188 aa of BC069480

Sequence: LRLVQPARLNPQVRPAVLPTRCPHPGEACVVSGWGLVSHNEPGTAGSPRSQVSLPDTLHCANISIISDTSCDKSYPGRLT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kallikrein-related peptidase 15
别名 ACO, ACO protease, HSRNASPH, Kallikrein 15, KLK15
计算分子量 255 aa, 28 kDa
观测分子量 28 kDa
GenBank蛋白编号BC069480
基因名称 KLK15
Gene ID (NCBI) 55554
RRIDAB_2880895
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H2R5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IHC protocol for KLK15 antibody 27515-1-APDownload protocol
WB protocol for KLK15 antibody 27515-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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