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验证数据展示

  • WB analysis using 21199-1-AP

    WB analysis using 21199-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21199-1-AP (KLHL35 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 21199-1-AP

    IHC staining of human heart using 21199-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21199-1-AP (KLHL35 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human heart using 21199-1-AP

    IHC staining of human heart using 21199-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart using 21199-1-AP (KLHL35 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 21199-1-AP

    IF Staining of HeLa using 21199-1-AP

    Immunofluorescent analysis of Hela cells, using KLHL35 antibody 21199-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of mouse heart using 21199-1-AP

    IHC staining of mouse heart using 21199-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21199-1-AP (KLHL35 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse heart using 21199-1-AP

    IHC staining of mouse heart using 21199-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21199-1-AP (KLHL35 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KLHL35 Polyclonal antibody

KLHL35 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21199-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

26597, kelch like 35 (Drosophila), Kelch like protein 35, KLHL35

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse kidney tissue, rat brain tissue, rat kidney tissue
Positive IHC detected inhuman heart tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21199-1-AP targets KLHL35 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KLHL35 fusion protein Ag15558 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch-like 35 (Drosophila)
别名 26597, kelch like 35 (Drosophila), Kelch like protein 35, KLHL35
计算分子量 363 aa, 40 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC042952
基因名称 KLHL35
Gene ID (NCBI) 283212
RRIDAB_10693685
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6PF15
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL35 antibody 21199-1-APDownload protocol
IHC protocol for KLHL35 antibody 21199-1-APDownload protocol
IF protocol for KLHL35 antibody 21199-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols