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验证数据展示

  • WB analysis using 21981-1-AP

    WB analysis using 21981-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21981-1-AP (KLHL34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 21981-1-AP

    IHC staining of human small intestine using 21981-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21981-1-AP (KLHL34 antibody) at dilution of 1:200 (under 10x lens).

KLHL34 Polyclonal antibody

KLHL34 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21981-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat, human

应用

WB, IHC, ELISA

别名

kelch like 34 (Drosophila), Kelch like protein 34, KLHL34

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21981-1-AP targets KLHL34 in WB, IHC, ELISA applications and shows reactivity with mouse, rat, human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 mouse, rat, human
免疫原 KLHL34 fusion protein Ag16001 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch-like 34 (Drosophila)
别名 kelch like 34 (Drosophila), Kelch like protein 34, KLHL34
计算分子量 644 aa, 71 kDa
观测分子量71 kDa
GenBank蛋白编号BC109031
基因名称 KLHL34
Gene ID (NCBI) 257240
RRIDAB_2878959
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N239
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLHL34 is a member of the Kelch-like (KLHL) gene family, which encodes a group of proteins that generally possess a BTB/POZ domain, a BACK domain and 5-6 Kelch motifs. KLHL genes are responsible for several Mendelian diseases and have been associated with cancer. The molecular mass of KLHL34 is 71 kDa.

实验方案

Product Specific Protocols
WB protocol for KLHL34 antibody 21981-1-APDownload protocol
IHC protocol for KLHL34 antibody 21981-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols