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KLHL34 Polyclonal antibody

KLHL34 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat, human

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 21981-1-AP

Print datasheet

Synonyms

kelch like 34 (Drosophila), Kelch like protein 34, KLHL34

验证数据展示

  • WB analysis using 21981-1-AP

    WB analysis using 21981-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21981-1-AP (KLHL34 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 21981-1-AP

    IHC staining of human small intestine using 21981-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21981-1-AP (KLHL34 antibody) at dilution of 1:200 (under 10x lens).

经过测试的应用

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21981-1-AP targets KLHL34 in WB, IHC, ELISA applications and shows reactivity with mouse, rat, human samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity mouse, rat, human
Immunogen KLHL34 fusion protein Ag16001 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kelch-like 34 (Drosophila)
Synonyms kelch like 34 (Drosophila), Kelch like protein 34, KLHL34
Calculated Molecular Weight 644 aa, 71 kDa
Observed Molecular Weight71 kDa
GenBank Accession NumberBC109031
Gene Symbol KLHL34
Gene ID (NCBI) 257240
RRIDAB_2878959
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N239
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLHL34 is a member of the Kelch-like (KLHL) gene family, which encodes a group of proteins that generally possess a BTB/POZ domain, a BACK domain and 5-6 Kelch motifs. KLHL genes are responsible for several Mendelian diseases and have been associated with cancer. The molecular mass of KLHL34 is 71 kDa.

实验方案

Product Specific Protocols
WB protocol for KLHL34 antibody 21981-1-APDownload protocol
IHC protocol for KLHL34 antibody 21981-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols