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验证数据展示

  • WB analysis of mouse embryo using 24781-1-AP

    WB analysis of mouse embryo using 24781-1-AP

    mouse embryo tissue were subjected to SDS PAGE followed by western blot with 24781-1-AP (KLHL31 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 24781-1-AP

    WB analysis using 24781-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 24781-1-AP (KLHL31 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 24781-1-AP

    IF Staining of HepG2 using 24781-1-AP

    Immunofluorescent analysis of HepG2 cells using 24781-1-AP (KLHL31 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

  • WB analysis of Jurkat using 24781-1-AP

    WB analysis of Jurkat using 24781-1-AP

    WB result of KLHL31 antibody (24781-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KLHL31 transfected Jurkat cells.

  • IHC staining of mouse skeletal muscle using 24781-1-AP

    IHC staining of mouse skeletal muscle using 24781-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24781-1-AP (KLHL31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse skeletal muscle using 24781-1-AP

    IHC staining of mouse skeletal muscle using 24781-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 24781-1-AP (KLHL31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • KD/KO验证

KLHL31 Polyclonal antibody

KLHL31 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24781-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

BKLHD6, KBTBD1, kelch like 31 (Drosophila), Kelch like protein 31, Kelch like protein KLHL, KLHL, KLHL31

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse embryo tissue, Jurkat cells
Positive IHC detected inmouse skeletal muscle tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24781-1-AP targets KLHL31 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20462

Product name: Recombinant human KLHL31 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-350 aa of BC137267

Sequence: MAPKKKIVKKNKGDINEMTIIVEDSPLNKLNALNGLLEGGNGLSCISSELTDASYGPNLLEGLSKMRQENFLCDLVIGTKTKSFDVHKSVMASCSEYFYNILKKDPSIQRVDLNDISPLGLATVIAYAYTGKLTLSLYTIGSIISAAVYLQIHTLIKMCSDFLIREMSVENCMYVVNIAETYSLKNAKAAAQKFIRDNFLEFAESDQFMKLTFEQINELLIDDDLQLPSEIVAFQIAMKWLEFDQKRVKYAADLLSNIRFGTISAQDLVNYVQSVPRMMQDADCHRLLVDAMNYHLLPYHQNTLQSRRTRIRGGCRVLVTVGGRPGLTEKSLSRDILYRDPENGWSKLTE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch-like 31 (Drosophila)
别名 BKLHD6, KBTBD1, kelch like 31 (Drosophila), Kelch like protein 31, Kelch like protein KLHL, KLHL, KLHL31
计算分子量 634 aa, 70 kDa
观测分子量 60-70 kDa
GenBank蛋白编号BC137267
基因名称 KLHL31
Gene ID (NCBI) 401265
RRIDAB_2879718
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H511
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLHL31 also named as BKLHD6, KBTBD1,or Kelch like protein 31 is a 634 amino acid protein, which contains one 1 BACK domain, one BTB domain and six Kelch repeats. KLHL31 as a transcription repressor in MAPK/JNK signaling pathway to regulate cellular functions. KLHL31 is strongly expressed in skeletal muscle and weakly in heart.

实验方案

Product Specific Protocols
IF protocol for KLHL31 antibody 24781-1-APDownload protocol
IHC protocol for KLHL31 antibody 24781-1-APDownload protocol
WB protocol for KLHL31 antibody 24781-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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