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验证数据展示

  • IHC staining of human liver using 25224-1-AP

    IHC staining of human liver using 25224-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 25224-1-AP

    IHC staining of human liver using 25224-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Jurkat using 25224-1-AP

    WB analysis of Jurkat using 25224-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 25224-1-AP (KLHL28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 25224-1-AP

    IHC staining of human liver using 25224-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25224-1-AP (KLHL28 Antibody) at dilution of 1:50 (under 40x lens).

KLHL28 Polyclonal antibody

KLHL28 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25224-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

BTBD5, kelch like 28 (Drosophila), Kelch like protein 28, KLHL28

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, mouse brain tissue
Positive IHC detected inhuman liver tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25224-1-AP targets KLHL28 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 KLHL28 fusion protein Ag18665 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch-like 28 (Drosophila)
别名 BTBD5, kelch like 28 (Drosophila), Kelch like protein 28, KLHL28
计算分子量 571 aa, 64 kDa
观测分子量 64 kDa
GenBank蛋白编号BC121009
基因名称 KLHL28
Gene ID (NCBI) 54813
RRIDAB_2879972
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NXS3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLHL28 also named as BTBD5 or Kelch like protein 28 is a 571 amino acid protein, which contains one BTB domain and six kelch repeats. KLHL28 localizes in the cytoplasm and belongs to BTB/kelch-associated proteins family. KLHL28 may play a role in organizing the actin cytoskeleton of the brain cells. KLHL28 is expressed as two isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for KLHL28 antibody 25224-1-APDownload protocol
IHC protocol for KLHL28 antibody 25224-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols