验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IP detected in | PC-3 cells |
Planning an IHC experiment? We recommend our IHCeasy KLHL22 Ready-To-Use IHC Kit. KLHL22 primary antibody included.
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:1000-1:6000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16214-1-AP targets KLHL22 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IP, ELISA Application Description |
文献引用应用 | WB, IP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse |
免疫原 | KLHL22 fusion protein Ag9212 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | kelch-like 22 (Drosophila) |
别名 | kelch like 22 (Drosophila), Kelch like protein 22, KELCHL, KLHL22 |
计算分子量 | 634 aa, 72 kDa |
观测分子量 | 72 kDa |
GenBank蛋白编号 | BC015923 |
基因名称 | KLHL22 |
Gene ID (NCBI) | 84861 |
RRID | AB_2131201 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q53GT1 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for KLHL22 antibody 16214-1-AP | Download protocol |
IP protocol for KLHL22 antibody 16214-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Nature KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing.
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J Cell Biol Usp16 regulates kinetochore localization of Plk1 to promote proper chromosome alignment in mitosis.
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Cell Rep SUMOylation Promotes Nuclear Import and Stabilization of Polo-like Kinase 1 to Support Its Mitotic Function.
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iScience Downregulation of UBE4B promotes CNS axon regrowth and functional recovery after stroke | ||
J Hum Genet A novel missense variant in CUL3 shows altered binding ability to BTB-adaptor proteins leading to diverse phenotypes of CUL3-related disorders. | ||
Neoplasma KLHL22 promotes malignant melanoma growth in vitro and in vivo by activating the PI3K/Akt/mTOR signaling pathway. |