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KLHL22 Polyclonal antibody

KLHL22 Polyclonal Antibody for WB, IP, ELISA
Cat No. 16214-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, ELISA

kelch like 22 (Drosophila), Kelch like protein 22, KELCHL, KLHL22

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy KLHL22 Ready-To-Use IHC Kit. KLHL22 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16214-1-AP targets KLHL22 in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 KLHL22 fusion protein Ag9212 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch-like 22 (Drosophila)
别名 kelch like 22 (Drosophila), Kelch like protein 22, KELCHL, KLHL22
计算分子量 634 aa, 72 kDa
观测分子量 72 kDa
GenBank蛋白编号BC015923
基因名称 KLHL22
Gene ID (NCBI) 84861
RRIDAB_2131201
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53GT1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL22 antibody 16214-1-APDownload protocol
IP protocol for KLHL22 antibody 16214-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nature

KLHL22 activates amino-acid-dependent mTORC1 signalling to promote tumorigenesis and ageing.

Authors - Jie Chen
  • KD Validated
humanWB

J Cell Biol

Usp16 regulates kinetochore localization of Plk1 to promote proper chromosome alignment in mitosis.

Authors - Xiaolong Zhuo
  • KD Validated
humanWB

Cell Rep

SUMOylation Promotes Nuclear Import and Stabilization of Polo-like Kinase 1 to Support Its Mitotic Function.

Authors - Donghua Wen
  • KD Validated
mouseWB

iScience

Downregulation of UBE4B promotes CNS axon regrowth and functional recovery after stroke

Authors - Shuang Jin
humanWB

J Hum Genet

A novel missense variant in CUL3 shows altered binding ability to BTB-adaptor proteins leading to diverse phenotypes of CUL3-related disorders.

Authors - Kohji Kato
humanWB

Neoplasma

KLHL22 promotes malignant melanoma growth in vitro and in vivo by activating the PI3K/Akt/mTOR signaling pathway.

Authors - X R Liu
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