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KLHL2 Polyclonal antibody

KLHL2 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 14449-1-AP

Print datasheet

Synonyms

ABP KELCH, Actin binding protein Mayven, Kelch like protein 2, KLHL2, MAV, MAYVEN

验证数据展示

  • WB analysis of MCF-7 using 14449-1-AP

    WB analysis of MCF-7 using 14449-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14449-1-AP (KLHL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 14449-1-AP

    WB analysis using 14449-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14449-1-AP (KLHL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 14449-1-AP

    IF Staining of MCF-7 using 14449-1-AP

    Immunofluorescent analysis of MCF-7 cells using 14449-1-AP (KLHL2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inK-562 cells, human brain tissue, MCF-7 cells, SH-SY5Y cells
Positive IF detected inMCF-7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14449-1-AP targets KLHL2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA
Tested Reactivity human, mouse, rat
Immunogen KLHL2 fusion protein Ag5950 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kelch-like 2, Mayven (Drosophila)
Synonyms ABP KELCH, Actin binding protein Mayven, Kelch like protein 2, KLHL2, MAV, MAYVEN
Calculated Molecular Weight 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC022503
Gene Symbol KLHL2
Gene ID (NCBI) 11275
RRIDAB_2234221
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95198
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL2 antibody 14449-1-APDownload protocol
IF protocol for KLHL2 antibody 14449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols