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验证数据展示

  • WB analysis of MCF-7 using 14449-1-AP

    WB analysis of MCF-7 using 14449-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14449-1-AP (KLHL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • WB analysis using 14449-1-AP

    WB analysis using 14449-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14449-1-AP (KLHL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 14449-1-AP

    IF Staining of MCF-7 using 14449-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using KLHL2 antibody (14449-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

KLHL2 Polyclonal antibody

KLHL2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 14449-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Kelch-like protein 2, Actin-binding protein Mayven, ABP KELCH

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inK-562 cells, human brain tissue, MCF-7 cells, SH-SY5Y cells
Positive IF/ICC detected inMCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14449-1-AP targets KLHL2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5950

Product name: Recombinant human KLHL2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 273-593 aa of BC022503

Sequence: DYLIEAMKYHLLPTEQRILMKSVRTRLRTPMNLPKLMVVVGGQAPKAIRSVECYDFKEERWHQVAELPSRRCRAGMVYMAGLVFAVGGFNGSLRVRTVDSYDPVKDQWTSVANMRDRRSTLGAAVLNGLLYAVGGFDGSTGLSSVEAYNIKSNEWFHVAPMNTRRSSVGVGVVGGLLYAVGGYDVASRQCLSTVECYNATTNEWTYIAEMSTRRSGAGVGVLNNLLYAVGGHDGPLVRKSVEVYDPTTNAWRQVADMNMCRRNAGVCAVNGLLYVVGGDDGSCNLASVEYYNPTTDKWTVVSSCMSTGRSYAGVTVIDKRL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch-like 2, Mayven (Drosophila)
别名 Kelch-like protein 2, Actin-binding protein Mayven, ABP KELCH
计算分子量 66 kDa
观测分子量 66 kDa
GenBank蛋白编号BC022503
基因名称 KLHL2
Gene ID (NCBI) 11275
RRIDAB_2234221
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95198
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KLHL2 contains an SH3-binding domain in the N terminus and a POZ domain and 6 tandem repeats of approximately 50 amino acids each in the C terminus. In astrocytoma/glioblastoma cells, KLHL2 colocalized with actin filaments in the cytoskeleton remodeling regions in stress fibers and in patchy cortical actin-rich regions of the cell margins. In primary rat hippocampal neurons, it was highly expressed in the cell body and in neurite processes(PMID: 10397770).

实验方案

Product Specific Protocols
IF protocol for KLHL2 antibody 14449-1-APDownload protocol
WB protocol for KLHL2 antibody 14449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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