• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of HepG2 using 17229-1-AP

    WB analysis of HepG2 using 17229-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 17229-1-AP

    IHC staining of human colon using 17229-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 17229-1-AP (KLHL18 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human colon using 17229-1-AP

    IHC staining of human colon using 17229-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 17229-1-AP (KLHL18 antibody) at dilution of 1:100 (under 40x lens).

  • Featured Product
  • KD/KO验证

KLHL18 Polyclonal antibody

KLHL18 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17229-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

OK/SW-cl.74, Kelch-like protein 18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inHepG2 cells, COLO 320 cells, HEK-293 cells, HeLa cells, HL-60 cells, mouse brain tissue
Positive IHC detected inhuman colon tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17229-1-AP targets KLHL18 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 KLHL18 fusion protein Ag11070 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kelch-like 18 (Drosophila)
别名 OK/SW-cl.74, Kelch-like protein 18
计算分子量 509 aa, 56 kDa
观测分子量 63 kDa, 56 kDa
GenBank蛋白编号BC032620
基因名称 KLHL18
Gene ID (NCBI) 23276
RRIDAB_10665554
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94889
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL18 antibody 17229-1-APDownload protocol
IHC protocol for KLHL18 antibody 17229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Biosci

KLHL18 inhibits the proliferation, migration, and invasion of non-small cell lung cancer by inhibiting PI3K/PD-L1 axis activity.

Authors - Xizi Jiang
View Article
humanWB

Biol Open

The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A.

Authors - Moghe Saili S
  • KD Validated
View Article