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  • Featured Product
  • KD/KO Validated

KLHL18 Polyclonal antibody

KLHL18 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 17229-1-AP

Print datasheet

Synonyms

kelch like 18 (Drosophila), Kelch like protein 18, KIAA0795, KLHL18

验证数据展示

  • WB analysis of HepG2 using 17229-1-AP

    WB analysis of HepG2 using 17229-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17229-1-AP (KLHL18 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon using 17229-1-AP

    IHC staining of human colon using 17229-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 17229-1-AP (KLHL18 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human colon using 17229-1-AP

    IHC staining of human colon using 17229-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon using 17229-1-AP (KLHL18 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 17229-1-AP

    IF Staining of HeLa using 17229-1-AP

    Immunofluorescent analysis of Hela cells, using KLHL18 antibody 17229-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inHepG2 cells, COLO 320 cells, HEK-293 cells, HeLa cells, HL-60 cells, mouse brain tissue
Positive IHC detected inhuman colon tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 17229-1-AP is KLHL18 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen KLHL18 fusion protein Ag11070 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kelch-like 18 (Drosophila)
Synonyms kelch like 18 (Drosophila), Kelch like protein 18, KIAA0795, KLHL18
Calculated Molecular Weight 509 aa, 56 kDa
Observed Molecular Weight 63 kDa, 56 kDa
GenBank Accession NumberBC032620
Gene Symbol KLHL18
Gene ID (NCBI) 23276
RRIDAB_10665554
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO94889
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLHL18 antibody 17229-1-APDownload protocol
IHC protocol for KLHL18 antibody 17229-1-APDownload protocol
IF protocol for KLHL18 antibody 17229-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Biosci

KLHL18 inhibits the proliferation, migration, and invasion of non-small cell lung cancer by inhibiting PI3K/PD-L1 axis activity.

Authors - Xizi Jiang
View Article
humanWB

Biol Open

The CUL3-KLHL18 ligase regulates mitotic entry and ubiquitylates Aurora-A.

Authors - Moghe Saili S
  • KD Validated
View Article