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验证数据展示

  • WB analysis using 83183-4-RR

    WB analysis using 83183-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83183-4-RR (KLF9 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of A549 using 83183-4-RR

    FC experiment of A549 using 83183-4-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug KLF9 Recombinant antibody (83183-4-RR, Clone:230436H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83183-4-RR

    Affinity and Kinetic Characterization of 83183-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83183-4-RR against Human KLF9 were performed. The affinity constant is 0.767 nM.

KLF9 Recombinant monoclonal antibody

KLF9 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83183-4-RR

产品说明书

CloneNo. 230436H2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), ELISA

别名

GC-box-binding protein 1, BTE-binding protein 1, BTEB1, BTEB, Basic transcription element-binding protein 1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, K-562 cells, HepG2 cells, A549 cells, U2OS cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83183-4-RR targets KLF9 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag34411

Product name: Recombinant human KLF9 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-150 aa of BC074880

Sequence: MSAAAYMDFVAAQCLVSISNRAAVPEHGVAPDAERLRLPEREVTKEHGDPGDTWKDYCTLVTIAKSLLDLNKYRPIQTPSVCSDSLESPDEDMGSDSDVTTESGSSPSHSPEERQDPGSAPSPLSLLHPGVAAKGKHASEKRHKCPYSGC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Kruppel-like factor 9
别名 GC-box-binding protein 1, BTE-binding protein 1, BTEB1, BTEB, Basic transcription element-binding protein 1
计算分子量27 kDa
观测分子量27-35 kDa
GenBank蛋白编号BC074880
基因名称 KLF9
Gene ID (NCBI) 687
RRIDAB_3670873
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13886
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Krüppel-like factor 9 (KLF9) was a member of SP/KLF family of DNA-binding transcriptional regulators featured by a highly homologous C-terminal three C2H2-type zinc finger DNA-binding domains. KLF9 has been previously reported to be downregulated in various types of human malignancy and serves as a tumour suppressor at tumour onset and development, affecting cell proliferation, apoptosis, metastasis and tumorigenicity (PMID: 32855660).

实验方案

Product Specific Protocols
WB protocol for KLF9 antibody 83183-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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