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验证数据展示

  • WB analysis of human skeletal muscle using 66185-1-Ig (same clone as 66185-1-PBS)

    WB analysis of human skeletal muscle using 66185-1-Ig (same clone as 66185-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66185-1-Ig (KLF15 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66185-1-PBS in a different storage buffer formulation.

  • WB analysis using 66185-1-Ig (same clone as 66185-1-PBS)

    WB analysis using 66185-1-Ig (same clone as 66185-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66185-1-Ig (KLF15 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66185-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 66185-1-Ig (same clone as 66185-1-PBS)

    IF Staining of HepG2 using 66185-1-Ig (same clone as 66185-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using KLF15 antibody (66185-1-Ig, Clone: 2B8G9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red). This data was developed using the same antibody clone with 66185-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

KLF15 Monoclonal antibody, PBS Only

KLF15 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66185-1-PBS

产品说明书

CloneNo. 2B8G9

宿主/亚型

Mouse / IgM

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

DKFZp779M1320, KKLF, KLF15, Krueppel like factor 15, Kruppel like factor 15

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66185-1-PBS targets KLF15 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4864

Product name: Recombinant human KLF15 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-416 aa of BC036733

Sequence: MVDHLLPVDENFSSPKCPVGYLGDRLVGRRAYHMLPSPVSEDDSDASSPCSCSSPDSQALCSCYGGGLGTESQDSILDFLLSQATLGSGGGSGSSIGASSGPVAWGPWRRAAAPVKGEHFCLPEFPLGDPDDVPRPFQPTLEEIEEFLEENMEPGVKEVPEGNSKDLDACSQLSAGPHKSHLHPGSSGRERCSPPPGGASAGGAQGPGGGPTPDGPIPVLLQIQPVPVKQESGTGPASPGQAPENVKVAQLLVNIQGQTFALVPQVVPSSNLNLPSKFVRIAPVPIAAKPVGSGPLGPGPAGLLMGQKFPKNPAAELIKMHKCTFPGCSKMYTKSSHLKAHLRRHTGEKPFACTWPGCGWRFSRSDELSRHRRSHSGVKPYQCPVCEKKFARSDHLSKHIKVHRFPRSSRSVRSVN

种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 Kruppel-like factor 15
别名 DKFZp779M1320, KKLF, KLF15, Krueppel like factor 15, Kruppel like factor 15
计算分子量 416 aa, 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC036733
基因名称 KLF15
Gene ID (NCBI) 28999
RRIDAB_2881580
偶联类型 Unconjugated
形式Liquid
纯化方式Thiophilic affinity chromatograph
UNIPROT IDQ9UIH9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KLF15, a kidney-enriched Krüppel-like zinc finger-containing transcription factors, is a transcriptional activator that binds the CLCNKA promoter. The nuclear protein KLF15, is expressed primarily in liver, heart, skeletal muscle and kidney tissues but is not detected in lymphoid tissues or bone marrow. It is an important regulator of GLUT4 in both adipose and muscle tissues.(PMID:10982849,PMID:16615998).

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