验证数据展示
经过测试的应用
| Positive WB detected in | MCF-7 cells, MDA-MB-231 cells, 3T3-L1 cells, C2C12 cells, mouse heart tissue, mouse kidney tissue, mouse liver tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13749-1-AP targets KLF15 in WB, IF, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB, IF, ChIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | KLF15 fusion protein Ag4690 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Kruppel-like factor 15 |
| 别名 | KKLF, KLF15, Krueppel like factor 15, Kruppel like factor 15 |
| 计算分子量 | 416 aa, 44 kDa |
| 观测分子量 | 44 kDa |
| GenBank蛋白编号 | BC036733 |
| 基因名称 | KLF15 |
| Gene ID (NCBI) | 28999 |
| RRID | AB_2132428 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9UIH9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KLF15, a kidney-enriched Krüppel-like zinc finger-containing transcription factors, is a transcriptonal activator that binds the CLCNKA promoter. The nuclear protein KLF15, is expressed primarily in liver, heart, skeletal muscle and kidney tissues but is not detected in lymphoid tissues or bone marrow. It is an important regulator of GLUT4 in both adipose and muscle tissues.Although the calculated molecular mass of KLF15 is 44 kDa, the apparent molecular mass on SDS-PAGE is 65 kDa due to the post-translational modifications (PMID:10982849,PMID:16615998).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KLF15 antibody 13749-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Crosstalk between glucocorticoid receptor and nutritional sensor mTOR in skeletal muscle. | ||
Mol Cell Biol A remodeled Hsp90 molecular chaperone ensemble with the novel co-chaperone Aarsd1 is required for muscle differentiation. | ||
Epigenomics Multi-omics insights into the pathogenesis of diabetic cardiomyopathy: epigenetic and metabolic profiles | ||