验证数据展示
经过测试的应用
| Positive WB detected in | mouse embryo tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| ChIP | See 1 publications below |
产品信息
34052-1-AP targets KLF11 in WB, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | ChIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag40584 Product name: Recombinant human KLF11 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-200 aa of BC063286 Sequence: MHTPDFAGPDDARAVDIMDICESILERKRHDSERSTCSILEQTDMEAVEALVCMSSWGQRSQKGDLLRIRPLTPVSDSGDVTTTVHMDAATPELPKDFHSLSTLCITPPQSPDLVEPSTRTPVSPQVTDSKACTATDVLQSSAVVARALSGGAERGLLGLEPVPSSPCRAKGTSVIRHTGESPAACFPTIQTPDCRLSDS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Kruppel-like factor 11 |
| 别名 | FKLF, FKLF1, Krueppel like factor 11, Kruppel like factor 11, MODY7 |
| 计算分子量 | 55 kDa |
| 观测分子量 | 65 kDa |
| GenBank蛋白编号 | BC063286 |
| 基因名称 | KLF11 |
| Gene ID (NCBI) | 8462 |
| RRID | AB_3743144 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O14901 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KLF11 (Krüppel-like factor 11) belongs to the zinc finger transcription factor family. Its C-terminus contains three tandem C2H2-type zinc finger domains responsible for sequence-specific DNA binding. It regulates transcription by binding to the GC box or CACCC element of target gene promoters, and in the pancreas, it negatively regulates insulin gene expression as a transcriptional repressor. Furthermore, KLF11 participates in cell cycle regulation, apoptosis, and fibrosis pathways, possesses tumor-suppressive functions.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KLF11 antibody 34052-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
