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KLC4 Polyclonal antibody

KLC4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 16493-1-AP

Print datasheet

Synonyms

kinesin light chain 4, Kinesin like protein 8, KLC 4, KLC4, KNSL8

验证数据展示

  • WB analysis using 16493-1-AP

    WB analysis using 16493-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16493-1-AP (KLC4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of human small intestine using 16493-1-AP

    IHC staining of human small intestine using 16493-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 16493-1-AP (KLC4 antibody) at dilution of 1:200 (under 10x lens).

经过测试的应用

Positive WB detected inA549 cells, NCI-H1299 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman small intestine tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16493-1-AP targets KLC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen KLC4 fusion protein Ag9621 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin light chain 4
Synonyms kinesin light chain 4, Kinesin like protein 8, KLC 4, KLC4, KNSL8
Calculated Molecular Weight 315 aa, 35 kDa
Observed Molecular Weight69 kDa
GenBank Accession NumberBC012357
Gene Symbol KLC4
Gene ID (NCBI) 89953
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NSK0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KLC4 antibody 16493-1-APDownload protocol
IHC protocol for KLC4 antibody 16493-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols