验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, NCI-H1299 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human small intestine tissue, human placenta tissue, human pancreas cancer tissue, human kidney tissue, rat pancreas tissue, rat liver tissue, mouse liver tissue, mouse pancreas tissue, mouse stomach tissue, rat stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16493-1-AP targets KLC4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag9621 Product name: Recombinant human KLC4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-315 aa of BC012357 Sequence: MSGLVLGQRDEPAGHRLSQEEILGSTRLVSQGLEALRSEHQAVLQSLSQTIECLQQGGHEEGLVHEKARQLRRSMENIELGLSEAQVMLALASHLSTVESEKQKLRAQVRRLCQENQWLRDELAGTQQRLQRSEQAVAQLEEEKKHLEFLGQLRQYDEDGHTSEEKEGDATKDSLDDLFPNEEEEDPSNGLSRGQGATAAQQGGYEIPARLRTLHNLVIQYAAQGRYEVAVPLCKQALEDLERTSGRGHPDVATMLNILALVYRDQNKYKEAAHLLNDALSIRESTLGPDHPAVSIPCPPHPTPRTPHHCCFGLS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kinesin light chain 4 |
| 别名 | Kinesin-like protein 8, KLC 4, KNSL8 |
| 计算分子量 | 315 aa, 35 kDa |
| 观测分子量 | 69 kDa |
| GenBank蛋白编号 | BC012357 |
| 基因名称 | KLC4 |
| Gene ID (NCBI) | 89953 |
| RRID | AB_2923563 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NSK0 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for KLC4 antibody 16493-1-AP | Download protocol |
| WB protocol for KLC4 antibody 16493-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |













