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验证数据展示

  • WB analysis using 22232-1-AP (same clone as 22232-1-PBS)

    WB analysis using 22232-1-AP (same clone as 22232-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 22232-1-AP (KIRREL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22232-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse kidney using 22232-1-AP (same clone as 22232-1-PBS)

    IF Staining of mouse kidney using 22232-1-AP (same clone as 22232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using KIRREL antibody (22232-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22232-1-PBS in a different storage buffer formulation.

KIRREL Polyclonal antibody, PBS Only

KIRREL Polyclonal Antibody for WB, IF-P, Indirect ELISA
Cat No. 22232-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF-P, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

22232-1-PBS targets KIRREL in WB, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag17578

Product name: Recombinant human KIRREL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 607-757 aa of BC109192

Sequence: NVRAHEDRPSSRAVLYADYRAPGPARFDGRPSSRLSHSSGYAQLNTYSRGPASDYGPEPTPPGPAAPAGTDTTSQLSYENYEKFNSHPFPGAAGYPTYRLGYPQAPPSGLERTPYEAYDPIGKYATATRFSYTSQHSDYGQRFQQRMQTHV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kin of IRRE like (Drosophila)
别名
计算分子量 757 aa, 84 kDa
观测分子量72 kDa
GenBank蛋白编号BC109192
基因名称 KIRREL
Gene ID (NCBI) 55243
RRIDAB_3085689
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96J84
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Kin of IRRE protein (KIRREL), also known as NEPH1, is a member of a podocin-binding protein family. KIRREL is a component of the podocyte slit diaphragm, which serves as the structural framework of the glomerular filtration barrier. KIRREL has three isoforms with molecular weights of 85 kDa, 83 kDa, and 72 kDa.

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