验证数据展示
经过测试的应用
| Positive WB detected in | HL-60 cells, NK-92 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32059-1-AP targets KIR2DL4/CD158d in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 4 |
| 别名 | KIR2DL4, KIR103, KIR 103AS, Killer cell immunoglobulin-like receptor 2DL4, G9P |
| 计算分子量 | 377 aa, 42 kDa |
| 观测分子量 | 45 kDa |
| GenBank蛋白编号 | BC041611 |
| 基因名称 | KIR2DL4 |
| Gene ID (NCBI) | 3805 |
| RRID | AB_3670180 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q99706 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells that regulate their responsiveness to signals received from their environment (PMID: 22934097). Killer cell immunoglobulin-like receptor 2DL4 (KIR2DL4, also known as CD158D and KIR103AS) is an unusual member of the KIRs family, which activates the cytotoxicity of NK cells. KIR2DL4 has two immunoglobulin domains and a single immunoreceptor tyrosine-based inhibition motif (ITIM) in the cytoplasmic tail with weak inhibitory potential (PMID: 12055234).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KIR2DL4/CD158d antibody 32059-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |