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验证数据展示

  • WB analysis of HeLa using 85981-1-RR (same clone as 85981-1-PBS)

    WB analysis of HeLa using 85981-1-RR (same clone as 85981-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 85981-1-RR (KIFC1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 85981-1-RR (same clone as 85981-1-PBS)

    IF Staining of HeLa using 85981-1-RR (same clone as 85981-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using KIFC1 antibody (85981-1-RR, Clone: 250588F10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 85981-1-RR (same clone as 85981-1-PBS)

    IHC staining of mouse testis using 85981-1-RR (same clone as 85981-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KIFC1 antibody included
  • IHC staining of mouse testis using 85981-1-RR (same clone as 85981-1-PBS)

    IHC staining of mouse testis using 85981-1-RR (same clone as 85981-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 85981-1-RR (KIFC1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85981-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KIFC1 antibody included

KIFC1 Recombinant antibody, PBS Only

KIFC1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85981-1-PBS

产品说明书

CloneNo. 250588F10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

HSET, Kinesin-like protein 2, Kinesin-like protein KIFC1, Kinesin-related protein HSET, KNSL2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy KIFC1 Ready-To-Use IHC Kit. KIFC1 primary antibody included.

产品信息

85981-1-PBS targets KIFC1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 KIFC1 fusion protein Ag14492 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kinesin family member C1
别名 HSET, Kinesin-like protein 2, Kinesin-like protein KIFC1, Kinesin-related protein HSET, KNSL2
计算分子量 673 aa, 74 kDa
观测分子量74 kDa
GenBank蛋白编号BC000712
基因名称 KIFC1
Gene ID (NCBI) 3833
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BW19
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KIFC1 is a molecular motor protein belonging to the kinesin-14 family that has motor domains at the C-terminus. KIFC1 is widely distributed, with highest expression in testis and participating in acrosome biogenesis and vesicle transport. It is also abundant in ovary, spleen, and liver. KIFC1 proteins are generally localized in the perinuclear region, and have been found to be involved chromosome alignment, congression and intracellular DNA transport (PMID: 12826589, PMID: 15236353, PMID: 23543461).