验证数据展示
经过测试的应用
| Positive WB detected in | human brain tissue, mouse testis tissue, mouse brain tissue, MCF-7 cells |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
12700-1-AP targets KIFAP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | KIFAP3 fusion protein Ag3394 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kinesin-associated protein 3 |
| 别名 | Kinesin-associated protein 3, kinesin associated protein 3, KIF3AP, KAP-3, KAP3 |
| 计算分子量 | 792 aa, 91 kDa |
| 观测分子量 | 91-100 kDa |
| GenBank蛋白编号 | BC028679 |
| 基因名称 | KIFAP3 |
| Gene ID (NCBI) | 22920 |
| RRID | AB_2132528 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92845 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIFAP3, also known as KIF3AP or KAP3, is a novel KIF3A/3B-associated protein. It binds to the tail domain of KIF3A/3B and may play a role in regulating the binding of KIF3A/3B to cargoes. Recently it has been reported that mutation within the KIFAP3 gene is associated with decreased KIFAP3 expression and increased survival in sporadic ALS, which makes it a potential target for ALS therapy. KIFAP3 has also been identified as the target of mir-130a.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KIFAP3 antibody 12700-1-AP | Download protocol |
| IHC protocol for KIFAP3 antibody 12700-1-AP | Download protocol |
| IF protocol for KIFAP3 antibody 12700-1-AP | Download protocol |
| IP protocol for KIFAP3 antibody 12700-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Commun Biol The germline factor DDX4 contributes to the chemoresistance of small cell lung cancer cells |
