验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse brain, mouse heart, rat brain |
Positive IP detected in | HepG2 cells |
Positive IHC detected in | human kidney tissue, human skin tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21632-1-AP targets KIF5B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF, IP, CoIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, pig, monkey |
免疫原 | KIF5B fusion protein Ag16250 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | kinesin family member 5B |
别名 | KIF5B, Kinesin 1 heavy chain, kinesin family member 5B, KINH, KNS, KNS1, Ubiquitous kinesin heavy chain, UKHC |
计算分子量 | 963 aa, 110 kDa |
观测分子量 | 110-120 kDa |
GenBank蛋白编号 | BC126279 |
基因名称 | KIF5B |
Gene ID (NCBI) | 3799 |
RRID | AB_11182931 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P33176 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIF5B, also known as KINH, KNS1, belongs to the Kinesin-1 family. KIF5B contains three structural domains: a globular N-terminal motor domain, a central alpha-helical rod domain, and a globular C-terminal domain. During the G2 phase of the cell cycle in a BICD2-dependent manner, KIF5B antagonizes the dynein function and drives the separation of nuclei and centrosomes (PMID: 20386726). KIF5B drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (KIF5B:24088571).
实验方案
Product Specific Protocols | |
---|---|
WB protocol for KIF5B antibody 21632-1-AP | Download protocol |
IHC protocol for KIF5B antibody 21632-1-AP | Download protocol |
IF protocol for KIF5B antibody 21632-1-AP | Download protocol |
IP protocol for KIF5B antibody 21632-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Arterioscler Thromb Vasc Biol Kinesin-1: A New Actor Involved in Platelet Secretion and Thrombus Stability.
| ||
J Hazard Mater Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria. | ||
J Cell Biol Kinesin-1 controls mast cell degranulation and anaphylaxis through PI3K-dependent recruitment to the granular Slp3/Rab27b complex.
| ||
EMBO Rep MICAL2PV suppresses the formation of tunneling nanotubes and modulates mitochondrial trafficking. | ||
Cancers (Basel) GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells. |