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KIF5B Polyclonal antibody

KIF5B Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21632-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, IP, ELISA and More (2)

KIF5B, Kinesin 1 heavy chain, kinesin family member 5B, KINH, KNS, KNS1, Ubiquitous kinesin heavy chain, UKHC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, Jurkat cells, mouse brain, mouse heart, rat brain
Positive IP detected inHepG2 cells
Positive IHC detected inhuman kidney tissue, human skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy KIF5B Ready-To-Use IHC Kit. KIF5B primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21632-1-AP targets KIF5B in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, monkey
免疫原 KIF5B fusion protein Ag16250 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 5B
别名 KIF5B, Kinesin 1 heavy chain, kinesin family member 5B, KINH, KNS, KNS1, Ubiquitous kinesin heavy chain, UKHC
计算分子量 963 aa, 110 kDa
观测分子量 110-120 kDa
GenBank蛋白编号BC126279
基因名称 KIF5B
Gene ID (NCBI) 3799
RRIDAB_11182931
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP33176
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF5B, also known as KINH, KNS1, belongs to the Kinesin-1 family. KIF5B contains three structural domains: a globular N-terminal motor domain, a central alpha-helical rod domain, and a globular C-terminal domain. During the G2 phase of the cell cycle in a BICD2-dependent manner, KIF5B antagonizes the dynein function and drives the separation of nuclei and centrosomes (PMID: 20386726). KIF5B drives the polarization of cytolytic granules and microtubule-organizing centers (MTOCs) toward the immune synapse between effector NK lymphocytes and target cells (KIF5B:24088571).

实验方案

Product Specific Protocols
WB protocol for KIF5B antibody 21632-1-APDownload protocol
IHC protocol for KIF5B antibody 21632-1-APDownload protocol
IF protocol for KIF5B antibody 21632-1-APDownload protocol
IP protocol for KIF5B antibody 21632-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF,IP

Arterioscler Thromb Vasc Biol

Kinesin-1: A New Actor Involved in Platelet Secretion and Thrombus Stability.

Authors - Frédéric Adam
  • KO Validated
ratIP

J Hazard Mater

Nano-graphene oxide depresses neurotransmission by blocking retrograde transport of mitochondria.

Authors - Yiyuan Kang
mouseWB,IF

J Cell Biol

Kinesin-1 controls mast cell degranulation and anaphylaxis through PI3K-dependent recruitment to the granular Slp3/Rab27b complex.

Authors - Isabelle Munoz
  • KO Validated
humanWB

EMBO Rep

MICAL2PV suppresses the formation of tunneling nanotubes and modulates mitochondrial trafficking.

Authors - Fei Wang
mouseIF

Elife

Brucella activates the host RIDD pathway to subvert BLOS1-directed immune defense.

Authors - Kelsey Michelle Wells
humanWB

Cancers (Basel)

GDF15 Is an Eribulin Response Biomarker also Required for Survival of DTP Breast Cancer Cells.

Authors - Chiara Bellio
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