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验证数据展示

  • WB analysis of mouse brain using 13711-1-AP

    WB analysis of mouse brain using 13711-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13711-1-AP (KIF2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 13711-1-AP

    IP experiment of mouse testis using 13711-1-AP

    IP result of anti-KIF2B (IP:13711-1-AP, 4ug; Detection:13711-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

  • IHC staining of mouse brain using 13711-1-AP

    IHC staining of mouse brain using 13711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13711-1-AP (KIF2B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF2B antibody included
  • IHC staining of mouse small intestine using 13711-1-AP

    IHC staining of mouse small intestine using 13711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 13711-1-AP (KIF2B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF2B antibody included
  • IHC staining of human placenta using 13711-1-AP

    IHC staining of human placenta using 13711-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 13711-1-AP (KIF2B antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF2B antibody included
  • IHC staining of mouse brain using 13711-1-AP

    IHC staining of mouse brain using 13711-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13711-1-AP (KIF2B antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with KIF2B antibody included

KIF2B Polyclonal antibody

KIF2B Polyclonal Antibody for WB, IP, ELISA
Cat No. 13711-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

Kinesin-like protein KIF2B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse testis tissue
Positive IP detected inmouse testis tissue
Planning an IHC experiment? We recommend our IHCeasy KIF2B Ready-To-Use IHC Kit. KIF2B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13711-1-AP targets KIF2B in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4648

Product name: Recombinant human KIF2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC033802

Sequence: MASQFCLPESPCLSPLKPLKPHFGDIQEGIYVAIQRSDKRIHLAVVTEINRENYWVTVEWVEKAVKKGKKIDLETILLLNPALDSAEHPMPPPPLSPLALAPSSAIRDQRTATKWVAMIPQKNQTASGDSLDVRVPSKPCLMKQKKSPCLWEIQKLQEQREKRRRLQQEIRARRALDVNTRNPNYEIMHMIEEYRRHLDSSKISVLEPPQEHRICVCVRKRPLNQRETTLKDLDIITVPSDNVVMVHESKQKVDLTRYLQNQTFCFDHAFDDKASNELVYQFTAQPLVESIFRKGMATCFAYGQTGSGKTYTMGGDFSGTAQDCSKGIYALVAQDVFLLLRNSTYEKLDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 2B
别名 Kinesin-like protein KIF2B
计算分子量 673 aa, 76 kDa
观测分子量 80 kDa
GenBank蛋白编号BC033802
基因名称 KIF2B
Gene ID (NCBI) 84643
RRIDAB_2131885
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N4N8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF2B belongs to the TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family and MCAK/KIF2 subfamily. KIF2B is evidently required for spindle assembly, chromosome movement and cytokinesis (PMID: 17538014). The molecular weight of KIF2B is 76 kDa. It plays an important role in chromosome congression (PMID: 23891108).

实验方案

Product Specific Protocols
IP protocol for KIF2B antibody 13711-1-APDownload protocol
WB protocol for KIF2B antibody 13711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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