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验证数据展示

  • WB analysis of mouse brain using 13711-1-AP

    WB analysis of mouse brain using 13711-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13711-1-AP (KIF2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 13711-1-AP

    IP experiment of mouse testis using 13711-1-AP

    IP result of anti-KIF2B (IP:13711-1-AP, 4ug; Detection:13711-1-AP 1:1000) with mouse testis tissue lysate 4000ug.

KIF2B Polyclonal antibody

KIF2B Polyclonal Antibody for WB, IP, ELISA
Cat No. 13711-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

KIF2B, kinesin family member 2B, Kinesin like protein KIF2B

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse testis tissue
Positive IP detected inmouse testis tissue
Planning an IHC experiment? We recommend our IHCeasy KIF2B Ready-To-Use IHC Kit. KIF2B primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13711-1-AP targets KIF2B in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag4648

Product name: Recombinant human KIF2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-350 aa of BC033802

Sequence: MASQFCLPESPCLSPLKPLKPHFGDIQEGIYVAIQRSDKRIHLAVVTEINRENYWVTVEWVEKAVKKGKKIDLETILLLNPALDSAEHPMPPPPLSPLALAPSSAIRDQRTATKWVAMIPQKNQTASGDSLDVRVPSKPCLMKQKKSPCLWEIQKLQEQREKRRRLQQEIRARRALDVNTRNPNYEIMHMIEEYRRHLDSSKISVLEPPQEHRICVCVRKRPLNQRETTLKDLDIITVPSDNVVMVHESKQKVDLTRYLQNQTFCFDHAFDDKASNELVYQFTAQPLVESIFRKGMATCFAYGQTGSGKTYTMGGDFSGTAQDCSKGIYALVAQDVFLLLRNSTYEKLDL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 2B
别名 KIF2B, kinesin family member 2B, Kinesin like protein KIF2B
计算分子量 673 aa, 76 kDa
观测分子量 80 kDa
GenBank蛋白编号BC033802
基因名称 KIF2B
Gene ID (NCBI) 84643
RRIDAB_2131885
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N4N8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF2B belongs to the TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family and MCAK/KIF2 subfamily. KIF2B is evidently required for spindle assembly, chromosome movement and cytokinesis (PMID: 17538014). The molecular weight of KIF2B is 76 kDa. It plays an important role in chromosome congression (PMID: 23891108).

实验方案

Product Specific Protocols
WB protocol for KIF2B antibody 13711-1-APDownload protocol
IP protocol for KIF2B antibody 13711-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols