验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse brain tissue, rat brain tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells, MDCK cells |
| Positive FC (Intra) detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
13105-1-AP targets KIF2A in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.
| 经测试应用 | WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IF, IP |
| 经测试反应性 | human, mouse, rat, canine |
| 文献引用反应性 | human, mouse |
| 免疫原 | KIF2A fusion protein Ag3748 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kinesin heavy chain member 2A |
| 别名 | Kinesin-2, Kinesin like protein KIF2A, kinesin heavy chain member 2A, Kinesin 2, KIF2 |
| 计算分子量 | 679 aa, 77 kDa |
| 观测分子量 | 80-100 kDa |
| GenBank蛋白编号 | BC031828 |
| 基因名称 | KIF2A |
| Gene ID (NCBI) | 3796 |
| RRID | AB_2265336 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O00139 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KIF2A antibody 13105-1-AP | Download protocol |
| IHC protocol for KIF2A antibody 13105-1-AP | Download protocol |
| IF protocol for KIF2A antibody 13105-1-AP | Download protocol |
| IP protocol for KIF2A antibody 13105-1-AP | Download protocol |
| FC protocol for KIF2A antibody 13105-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
EMBO J The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network
| ||
J Cell Biol WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin. | ||
EMBO Rep Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis | ||
Front Oncol Identification of Glycolysis-Related lncRNAs and the Novel lncRNA WAC-AS1 Promotes Glycolysis and Tumor Progression in Hepatocellular Carcinoma. | ||
Biomed Pharmacother KIF2A promotes the progression via AKT signaling pathway and is upregulated by transcription factor ETV4 in human gastric cancer.
| ||
Mol Biol Cell Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment. |
