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KIF2A Polyclonal antibody

KIF2A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 13105-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat, canine

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

HK2, KIF2, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells, MDCK cells
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13105-1-AP targets KIF2A in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse, rat, canine
文献引用反应性human, mouse
免疫原 KIF2A fusion protein Ag3748 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin heavy chain member 2A
别名 HK2, KIF2, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A
计算分子量 679 aa, 77 kDa
观测分子量 80-100 kDa
GenBank蛋白编号BC031828
基因名称 KIF2A
Gene ID (NCBI) 3796
RRIDAB_2265336
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO00139
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kinesin family member 2a (KIF2A), one of the kinesin family members, is identified in mammalian cells and involved in regulating microtubule dynamics during the mitosis via modulating intracellular transport, cell division, and bipolar spindle assembly (PMID: 17538014). High expression of KIF2A correlates with advanced tumor stages and worse prognosis in non-small cell lung cancer patients (PMID: 31858647). Within clinical practice, KIF2A exhibits key prognostic value for the management of gastric cancer, colorectal cancer and nasopharyngeal carcinoma (PMID: 32106376, PMID: 26070867, PMID: 26602436).

实验方案

Product Specific Protocols
WB protocol for KIF2A antibody 13105-1-APDownload protocol
IHC protocol for KIF2A antibody 13105-1-APDownload protocol
IF protocol for KIF2A antibody 13105-1-APDownload protocol
IP protocol for KIF2A antibody 13105-1-APDownload protocol
FC protocol for KIF2A antibody 13105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

EMBO J

The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network

Authors - Cuirong Guan
  • KD Validated
humanWB,IF

J Cell Biol

WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin.

Authors - Junjie Huang
mouseWB,IF

EMBO Rep

Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis

Authors - Jia-Qian Ju
humanWB

Front Oncol

Identification of Glycolysis-Related lncRNAs and the Novel lncRNA WAC-AS1 Promotes Glycolysis and Tumor Progression in Hepatocellular Carcinoma.

Authors - Xigang Xia
humanWB

Biomed Pharmacother

KIF2A promotes the progression via AKT signaling pathway and is upregulated by transcription factor ETV4 in human gastric cancer.

Authors - Xin Zhang
  • KD Validated
humanIF

Mol Biol Cell

Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment.

Authors - Hongdan Yang
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