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  • Featured Product
  • KD/KO Validated

KIF2A Polyclonal antibody

KIF2A Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, Canine

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 13105-1-AP

Print datasheet

Synonyms

HK2, KIF2, KIF2A, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A, KNS2

验证数据展示

  • WB analysis of HeLa using 13105-1-AP

    WB analysis of HeLa using 13105-1-AP

    WB result of KIF2A antibody (13105-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF2A transfected HeLa cells.

  • WB analysis using 13105-1-AP

    WB analysis using 13105-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13105-1-AP (KIF2A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IF Staining of A549 using 13105-1-AP

    IF Staining of A549 using 13105-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using KIF2A antibody (13105-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • FC experiment of A549 using 13105-1-AP

    FC experiment of A549 using 13105-1-AP

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human KIF2A (13105-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IP experiment of mouse brain using 13105-1-AP

    IP experiment of mouse brain using 13105-1-AP

    IP result of anti-KIF2A (IP:13105-1-AP, 4ug; Detection:13105-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

  • IHC staining of human breast cancer using 13105-1-AP

    IHC staining of human breast cancer using 13105-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human breast cancer using 13105-1-AP

    IHC staining of human breast cancer using 13105-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13105-1-AP (KIF2A Antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells, MDCK cells
Positive FC detected inA549 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:250-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13105-1-AP targets KIF2A in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat, Canine samples.

Tested Applications FC, IF/ICC, IHC, IP, WB, ELISA
Cited ApplicationsWB, IF
Tested Reactivity human, mouse, rat, Canine
Cited Reactivityhuman, mouse
Immunogen KIF2A fusion protein Ag3748 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin heavy chain member 2A
Synonyms HK2, KIF2, KIF2A, Kinesin 2, kinesin heavy chain member 2A, Kinesin like protein KIF2A, KNS2
Calculated Molecular Weight 679 aa, 77 kDa
Observed Molecular Weight 80-100 kDa
GenBank Accession NumberBC031828
Gene Symbol KIF2A
Gene ID (NCBI) 3796
RRIDAB_2265336
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00139
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIF2A antibody 13105-1-APDownload protocol
IHC protocol for KIF2A antibody 13105-1-APDownload protocol
IF protocol for KIF2A antibody 13105-1-APDownload protocol
IP protocol for KIF2A antibody 13105-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB, IF

J Cell Biol

WDR62 regulates spindle dynamics as an adaptor protein between TPX2/Aurora A and katanin.

Authors - Junjie Huang
View Article
mouseWB,IF

EMBO Rep

Mcrs1 regulates G2/M transition and spindle assembly during mouse oocyte meiosis

Authors - Jia-Qian Ju
View Article
humanWB

Front Oncol

Identification of Glycolysis-Related lncRNAs and the Novel lncRNA WAC-AS1 Promotes Glycolysis and Tumor Progression in Hepatocellular Carcinoma.

Authors - Xigang Xia
View Article
humanWB

Biomed Pharmacother

KIF2A promotes the progression via AKT signaling pathway and is upregulated by transcription factor ETV4 in human gastric cancer.

Authors - Xin Zhang
  • KD Validated
View Article
humanIF

Mol Biol Cell

Mps1 regulates spindle morphology through MCRS1 to promote chromosome alignment.

Authors - Hongdan Yang
View Article
humanWB

Biochem Biophys Res Commun

High KIF2A expression promotes proliferation, migration and predicts poor prognosis in lung adenocarcinoma.

Authors - Tian Xie
  • KD Validated
View Article