验证数据展示
经过测试的应用
| Positive WB detected in | MDCK cells, HEK-293 cells, MCF-7 cells |
| Positive IHC detected in | human kidney tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17422-1-AP targets KIF26B in WB, IHC, IF, ELISA applications and shows reactivity with human, canine samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, canine |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag11446 Product name: Recombinant human KIF26B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 151-355 aa of BC042481 Sequence: AFSAVIHDKLQVPNTIRKAWNDRDNRCDICATHLNQLKQEAIQMVLTLEQAAGSEHYDASPCSPPPLSNIPTLVGSRHVGGLQQPRDWAFVPAPCATSNYTGFANKHGSKPSSLGVSNGAEKKSGSPTHQAKVSLQMATSPSNGNILNSVAIQAHQYLDGTWSLSRTNGVTLYPYQDSEALVTRGRGRLEDSAAAPALMSEDRGA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kinesin family member 26B |
| 别名 | Kinesin like protein KIF26B, Kinesin-like protein KIF26B |
| 计算分子量 | 2108aa,224 kDa; 162aa,17 kDa |
| 观测分子量 | 224-230 kDa |
| GenBank蛋白编号 | BC042481 |
| 基因名称 | KIF26B |
| Gene ID (NCBI) | 55083 |
| RRID | AB_2131762 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q2KJY2 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIF26B is a member of kinesin-11 family and plays important role in embryogenesis. It is a downstram target of Sall1 and is essential for kidney development. KIF26B is also involved in tumorigenesis.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for KIF26B antibody 17422-1-AP | Download protocol |
| WB protocol for KIF26B antibody 17422-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Fusobacterium nucleatum reduces METTL3-mediated m6A modification and contributes to colorectal cancer metastasis.
| ||
Biomed Pharmacother KIF26B promotes cell proliferation and migration through the FGF2/ERK signaling pathway in breast cancer. | ||
J Bone Miner Res KIF26B Silencing Prevents Osseous Transdifferentiation of Progenitor/Stem Cells and Attenuates Ectopic Calcification in a Murine Model.
| ||
J Ginseng Res Ginsenoside Rh2 reduces m6A RNA methylation in cancer via the KIF26B-SRF positive feedback loop | ||
Cell Cycle Noncoding RNAs-based high KIF26B expression correlates with poor prognosis and tumor immune infiltration in colon cancer | ||
Biochem Biophys Res Commun Novel mechanistic role of Kif26b in adipogenic differentiation of murine multipotent stromal cells.
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