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  • KD/KO Validated

KIF23 Polyclonal antibody

KIF23 Polyclonal Antibody for WB, IF/ICC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IF/ICC, IHC, ELISA

Conjugate

Unconjugated

Cat No : 28587-1-AP

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Synonyms

CHO1, KIF23, kinesin family member 23, Kinesin like protein 5, Kinesin like protein KIF23, KNSL5, Mitotic kinesin like protein 1, MKLP 1, MKLP1



经过测试的应用

Positive WB detected inHeLa cells, U-87 MG cells
Positive IHC detected inhuman stomach cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28587-1-AP targets KIF23 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, IHC, ELISA
Cited ApplicationsWB, IF, IHC
Tested Reactivity Human
Cited Reactivityhuman
Immunogen KIF23 fusion protein Ag29346 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin family member 23
Synonyms CHO1, KIF23, kinesin family member 23, Kinesin like protein 5, Kinesin like protein KIF23, KNSL5, Mitotic kinesin like protein 1, MKLP 1, MKLP1
Calculated Molecular Weight 110 kDa
Observed Molecular Weight110 kDa
GenBank Accession NumberNM_138555
Gene Symbol KIF23
Gene ID (NCBI) 9493
RRIDAB_2881176
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02241
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIF23 antibody 28587-1-APDownload protocol
IHC protocol for KIF23 antibody 28587-1-APDownload protocol
IF protocol for KIF23 antibody 28587-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

PLoS Biol

The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.

Authors - Umut Batman
humanWB,IHC

Front Cell Dev Biol

Identification of KIF23 as a Prognostic Biomarker Associated With Progression of Clear Cell Renal Cell Carcinoma.

Authors - Zonglong Wu
  • KD Validated
humanWB

Front Immunol

HBx Mediated Increase of DDX17 Contributes to HBV-Related Hepatocellular Carcinoma Tumorigenesis.

Authors - Mei-Ling Dong
humanWB

Toxicol Appl Pharmacol

Kinesin family member 23 exerts a protumor function in breast cancer via stimulation of the Wnt/β-catenin pathway.

Authors - Xin He
  • KD Validated
WB

Cell Rep

Augmin-dependent microtubule self-organization drives kinetochore fiber maturation in mammals.

Authors - Ana C Almeida
  • KD Validated