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KIF19 Polyclonal antibody

KIF19 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB,ELISA and More (1)

Conjugate

Unconjugated

Cat No : 19551-1-AP

Print datasheet

Synonyms

KIF19, KIF19A, kinesin family member 19, Kinesin like protein KIF19

验证数据展示

  • WB analysis of human brain using 19551-1-AP

    WB analysis of human brain using 19551-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19551-1-AP (KIF19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 19551-1-AP

    IHC staining of human brain using 19551-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19551-1-AP (KIF19 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 19551-1-AP

    IHC staining of human brain using 19551-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19551-1-AP (KIF19 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 19551-1-AP

    IF Staining of HepG2 using 19551-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIF19 antibody 19551-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19551-1-AP targets KIF19 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IHC, WB,ELISA
Cited ApplicationsWB, IP, IF
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name kinesin family member 19
Synonyms KIF19, KIF19A, kinesin family member 19, Kinesin like protein KIF19
Calculated Molecular Weight 111 kDa
Observed Molecular Weight 92 kDa
GenBank Accession NumberNM_153209
Gene Symbol KIF19
Gene ID (NCBI) 124602
RRIDAB_10640802
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ2TAC6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF19 belongs to the kinesin-like protein family. The antibody recognizes isoform1 of KIF19 only.

实验方案

Product Specific Protocols
WB protocol for KIF19 antibody 19551-1-APDownload protocol
IHC protocol for KIF19 antibody 19551-1-APDownload protocol
IF protocol for KIF19 antibody 19551-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Mol Cell Biol

Production of transgenic mice by random recombination of targeted genes in female germline stem cells.

Authors - Zhang Yong Y
View Article
human,mouseWB,IF,IP

J Biol Chem

AC6 regulates the microtubule-depolymerizing kinesin KIF19A to control ciliary length in mammals.

Authors - Kavisha Arora
View Article