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验证数据展示

  • WB analysis of human brain using 19551-1-AP

    WB analysis of human brain using 19551-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 19551-1-AP (KIF19 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 19551-1-AP

    IHC staining of human brain using 19551-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19551-1-AP (KIF19 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human brain using 19551-1-AP

    IHC staining of human brain using 19551-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 19551-1-AP (KIF19 antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 19551-1-AP

    IF Staining of HepG2 using 19551-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIF19 antibody 19551-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

KIF19 Polyclonal antibody

KIF19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19551-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA and More (2)

别名

Kinesin-like protein KIF19

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

19551-1-AP targets KIF19 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IP, IF
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 19
别名 Kinesin-like protein KIF19
计算分子量 111 kDa
观测分子量 92 kDa
GenBank蛋白编号NM_153209
基因名称 KIF19
Gene ID (NCBI) 124602
RRIDAB_10640802
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ2TAC6
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF19 belongs to the kinesin-like protein family. The antibody recognizes isoform1 of KIF19 only.

实验方案

Product Specific Protocols
WB protocol for KIF19 antibody 19551-1-APDownload protocol
IHC protocol for KIF19 antibody 19551-1-APDownload protocol
IF protocol for KIF19 antibody 19551-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Mol Cell Biol

Production of transgenic mice by random recombination of targeted genes in female germline stem cells.

Authors - Zhang Yong Y
View Article
human,mouseWB,IF,IP

J Biol Chem

AC6 regulates the microtubule-depolymerizing kinesin KIF19A to control ciliary length in mammals.

Authors - Kavisha Arora
View Article