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验证数据展示

  • IP experiment of Jurkat using 33139-1-AP

    IP experiment of Jurkat using 33139-1-AP

    IP result of anti-KIF18B (IP:33139-1-AP, 4ug; Detection:33139-1-AP 1:400) with Jurkat cells lysate 1360 ug.

  • IF Staining of Jurkat using 33139-1-AP

    IF Staining of Jurkat using 33139-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using KIF18B antibody (33139-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of U2OS using 33139-1-AP

    IF Staining of U2OS using 33139-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using KIF18B antibody (33139-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

KIF18B Polyclonal antibody

KIF18B Polyclonal Antibody for IF/ICC, IP, ELISA
Cat No. 33139-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, IP, ELISA

别名

Kinesin-like protein KIF18B

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IP detected inJurkat cells
Positive IF/ICC detected inU2OS cells, Jurkat cells

推荐稀释比

应用推荐稀释比
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33139-1-AP targets KIF18B in IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag36663

Product name: Recombinant human KIF18B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-110 aa of BC136590

Sequence: MAVEDSTLQVVVRVRPPTPRELDSQRRPVVQVVDERVLVFNPEEPDGGFPGLKWGGTHDGPKKKGKDLTFVFDRVFGEAATQQDVFQHTTHSVLDSFLQGYNCSVFAYGA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinesin family member 18B
别名 Kinesin-like protein KIF18B
计算分子量852 aa, 93 kDa/833 aa, 91 kDa
观测分子量100 kDa
GenBank蛋白编号BC136590
基因名称 KIF18B
Gene ID (NCBI) 146909
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ86Y91
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF18B is a key member of the kinesin-8 family, involved in regulating various physiological processes such as microtubule length, spindle assembly, and chromosome alignment. This article briefly introduces the structure and physiological functions of KIF18B, examines its role in malignant tumors, and the associated carcinogenic signaling pathways such as PI3K/AKT, Wnt/β-catenin, and mTOR pathways. Research indicates that the upregulation of KIF18B enhances tumor malignancy and resistance to radiotherapy and chemotherapy. KIF18B could become a new target for anticancer drugs, offering significant potential for the treatment of malignant tumors and reducing chemotherapy resistance (PMID: 39259333).

实验方案

Product Specific Protocols
IF protocol for KIF18B antibody 33139-1-APDownload protocol
IP protocol for KIF18B antibody 33139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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