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验证数据展示

  • WB analysis using 85046-1-RR

    WB analysis using 85046-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85046-1-RR (KIF17 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 85046-1-RR

    IHC staining of mouse brain using 85046-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85046-1-RR (KIF17 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 85046-1-RR

    IF Staining of mouse brain using 85046-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using KIF17 antibody (85046-1-RR, Clone: 242469G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85046-1-RR

    Affinity and Kinetic Characterization of 85046-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85046-1-RR against Human KIF17 were performed. The affinity constant is below 1 pM.

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KIF17 Recombinant monoclonal antibody

KIF17 Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 85046-1-RR

产品说明书

CloneNo. 242469G4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, ELISA

别名

242469G4, KIAA1405, KIF3-related motor protein, KIF3X, Kinesin-like protein KIF17

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85046-1-RR targets KIF17 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6217

Product name: Recombinant human KIF17 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-351 aa of BC065927

Sequence: MASEAVKVVVRCRPMNQRERELRCQPVVTVDCARAQCCIQNPGAADEPPKQFTFDGAYHVDHVTEQIYNEIAYPLVEGVTEGYNGTIFAYGQTGSGKSFTMQGLPDPPSQRGIIPRAFEHVFESVQCAENTKFLVRASYLEIYNEDVRDLLGADTKQKLELKEHPEKGVYVKGLSMHTVHSVAQCEHIMETGWKNRSVGYTLMNKDSSRSHSIFTISIEMSAVDERGKDHLRAGKLNLVDLAGSERQSKTGATGERLKEATKINLSLSALGNVISALVDGRCKHVPYRDSKLTRLLQDSLGGNTKTLMVACLSPADNNYDETLSTLRYANRAKNIRNKPRINEDPKDALLR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kinesin family member 17
别名 242469G4, KIAA1405, KIF3-related motor protein, KIF3X, Kinesin-like protein KIF17
计算分子量 115 kDa
观测分子量 170 kDa
GenBank蛋白编号BC065927
基因名称 KIF17
Gene ID (NCBI) 57576
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9P2E2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIF17 is a kinesin superfamily member, encoding a neuron-specific molecular motor. In neurons, KIF17 is responsible for the specialized transport of the N-methyl-D-aspartate receptor NR2B subunit, the glutamate receptor GluR5, and the potassium channel Kv4.2 from the cell body to the dendrites(PMID: 23762210).

实验方案

Product Specific Protocols
WB protocol for KIF17 antibody 85046-1-RRDownload protocol
IHC protocol for KIF17 antibody 85046-1-RRDownload protocol
IF protocol for KIF17 antibody 85046-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols