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验证数据展示

  • WB analysis using 82903-1-RR (same clone as 82903-1-PBS)

    WB analysis using 82903-1-RR (same clone as 82903-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82903-1-RR (KIF15 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82903-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 82903-1-RR (same clone as 82903-1-PBS)

    WB analysis of HeLa using 82903-1-RR (same clone as 82903-1-PBS)

    WB result of KIF15 antibody (82903-1-RR; 1:8000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIF15 transfected HeLa cells. This data was developed using the same antibody clone with 82903-1-PBS in a different storage buffer formulation.

  • IHC staining of human intrahepatic cholangiocarcinoma using 82903-1-RR (same clone as 82903-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 82903-1-RR (same clone as 82903-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 82903-1-RR (KIF15 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82903-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with KIF15 antibody included
  • Affinity and Kinetic Characterization of 82903-1-RR

    Affinity and Kinetic Characterization of 82903-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82903-1-RR against Human KIF15 were performed. The affinity constant is 75.8 pM.

  • Featured Product
  • KD/KO验证

KIF15 Recombinant monoclonal antibody, PBS Only

KIF15 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 82903-1-PBS

产品说明书

CloneNo. 230049F1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

230049F1, hKLP2, Kinesin-like protein 2, Kinesin-like protein 7, Kinesin-like protein KIF15

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy KIF15 Ready-To-Use IHC Kit. KIF15 primary antibody included.

产品信息

82903-1-PBS targets KIF15 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag33897

Product name: Recombinant human KIF15 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 950-1050 aa of NM_020242

Sequence: EQQMAKVQKLEESLLATEKVISSLEKSRDSDKKVVADLMNQIQELRTSVCEKTETIDTLKQELKDINCKYNSALVDREESRVLIKKQEVDILDLKETLRLR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 kinesin family member 15
别名 230049F1, hKLP2, Kinesin-like protein 2, Kinesin-like protein 7, Kinesin-like protein KIF15
计算分子量160 kDa
观测分子量160 kDa
GenBank蛋白编号NM_020242
基因名称 KIF15
Gene ID (NCBI) 56992
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9NS87
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)

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