验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse thymus tissue, HEK-293 cells, MDA-MB-453s cells |
| Positive IP detected in | mouse thymus tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HEK-293 cells |
Planning an IHC experiment? We recommend our IHCeasy KIF15 Ready-To-Use IHC Kit. KIF15 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55407-1-AP targets KIF15 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, FC (Intra), IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, zebrafish |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kinesin family member 15 |
| 别名 | hKLP2, Kinesin-like protein 2, Kinesin-like protein 7, Kinesin-like protein KIF15, KLP2 |
| 计算分子量 | 160 kDa |
| 观测分子量 | 150-165 kDa |
| GenBank蛋白编号 | NM_020242 |
| 基因名称 | KIF15 |
| Gene ID (NCBI) | 56992 |
| RRID | AB_11182836 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9NS87 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIF15, also named as KLP2 and KNSL7, belongs to the kinesin-like protein family and KLP2 subfamily. It is a plus-end directed kinesin-like motor enzyme which involved in mitotic spindle assembly. Several isoforms of KIF15 exist due to the alternative splicing, with molecular weight between 130-160 kDa. Sometimes higher molecular weight around 200 kDa can also be observed, which may be a modified variant of KIF15. (14618103)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for KIF15 antibody 55407-1-AP | Download protocol |
| IHC protocol for KIF15 antibody 55407-1-AP | Download protocol |
| IP protocol for KIF15 antibody 55407-1-AP | Download protocol |
| WB protocol for KIF15 antibody 55407-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancer Res KIF15-mediated stabilization of AR and AR-V7 contributes to enzalutamide resistance in prostate cancer.
| ||
Am J Respir Crit Care Med Rare and Common Variants in KIF15 Contribute to Genetic Risk of Idiopathic Pulmonary Fibrosis | ||
Cell Death Dis B7-H3 regulates KIF15-activated ERK1/2 pathway and contributes to radioresistance in colorectal cancer.
| ||
Int J Biol Sci ZNF367-induced transcriptional activation of KIF15 accelerates the progression of breast cancer. | ||
Int J Biol Sci KIF15 promotes human glioblastoma progression under the synergistic transactivation of REST and P300
| ||
J Transl Med Inhibition of the FEN1-PBX1 axis elicits cellular senescence in breast cancer via the increased intracellular reactive oxygen species levels |
