验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue, Y79 cells, mouse pancreas tissue, rat kidney tissue, mouse heart tissue |
| Positive IHC detected in | human kidney tissue, human pancreas cancer tissue, human renal cell carcinoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12035-1-AP targets KIF12 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag2707 Product name: Recombinant human KIF12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-334 aa of BC010626 Sequence: MQRTFAWLLDRVQHLGAPVTLRASYLEIYNEQVRDLLSLGSPRPLPVRWNKTRGFYVEQLRVVEFGSLEALMELLQTGLSRRRNSAHTLNQASSRSHALLTLYISRQTAQQMPSVDPGEPPVGGKLCFVDLAGSEKVAATGSRGELMLEANSINRSLLALGHCISLLLDPQRKQSHIPFRDSKLTKLLADSLGGRGVTLMVACVSPSAQCLPETLSTLRYASRAQRVTTRPQAPKSPVAKQPQRLETEMLQLQEENRRLQFQLDQMDCKASGLSGARVAWAQRNLYGMLQEFMLENERLRKEKSQLQNSRDLAQNEQRILAQQVHALERRLLSA 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | kinesin family member 12 |
| 别名 | Kinesin like protein KIF12, Kinesin-like protein KIF12, RP11 56P10.3 |
| 计算分子量 | 646 aa, 71 kDa |
| 观测分子量 | 55-70 kDa |
| GenBank蛋白编号 | BC010626 |
| 基因名称 | KIF12 |
| Gene ID (NCBI) | 113220 |
| RRID | AB_2280813 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96FN5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIF12(kinesin family member 12) is a member of the kinesin superfamily (KIF), which is a microtubule-dependent ATP-driven molecular motor, and its core is involved in the regulation of organelle transport, cell division and metabolic enzyme degradation. Its functional defects are closely related to bile duct diseases, liver diseases and tumors.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for KIF12 antibody 12035-1-AP | Download protocol |
| WB protocol for KIF12 antibody 12035-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Kidney Int Copy number variation analysis identifies novel CAKUT candidate genes in children with a solitary functioning kidney. | ||
Breast Cancer Res Treat Specific kinesin expression profiles associated with taxane resistance in basal-like breast cancer. | ||
J Proteomics Proteomic analysis of podocyte exosome-enriched fraction from normal human urine. | ||
J Pediatr KIF12 Variants and Disturbed Hepatocyte Polarity in Children With a Phenotypic Spectrum of Cholestatic Liver Disease. |
