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KIDINS220 Polyclonal antibody

KIDINS220 Polyclonal Antibody for WB, IP, ELISA
Cat No. 21856-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IP, ELISA and More (1)

Ankyrin repeat-rich membrane-spanning protein, ARMS, KIDINS 220, Kinase D-interacting substrate of 220 kDa

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, human brain tissue, Raji cells
Positive IP detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21856-1-AP targets KIDINS220 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IP, ELISA Application Description
文献引用应用WB, IHC, IP
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原

CatNo: Ag16448

Product name: Recombinant human KIDINS220 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1374-1672 aa of BC130610

Sequence: LDPITEEDEKSDQSGSKLLPGKKSSERSSLFQTDLKLKGSGLRYQKLPSDEDESGTEESDNTPLLKDDKDRKAEGKVERVPKSPEHSAEPIRTFIKAKEYLSDALLDKKDSSDSGVRSSESSPNHSLHNEVADDSQLEKANLIELEDDSHSGKRGIPHSLSGLQDPIIARMSICSEDKKSPSECSLIASSPEENWPACQKAYNLNRTPSTVTLNNNSAPANRANQNFDEMEGIRETSQVILRPSSSPNPTTIQNENLKSMTHKRSQRSSYTRLSKDPPELHAAASSESTGFGEERESIL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 kinase D-interacting substrate, 220kDa
别名 Ankyrin repeat-rich membrane-spanning protein, ARMS, KIDINS 220, Kinase D-interacting substrate of 220 kDa
计算分子量 1672 aa, 187 kDa
观测分子量 186-197 kDa
GenBank蛋白编号BC130610
基因名称 KIDINS220
Gene ID (NCBI) 57498
RRIDAB_10836934
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9ULH0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Kidins220 (Kinase D-interacting substrate 220 kD) is a crucial mediator of signal transduction in neural tissues. Also it named ARMS(Ankyrin Repeat-Rich Membrane Spanning) due to the presence of ankyrin repeats and transmembrane domains together with other binding motifs. It is a multidomain transmembrane protein that not only transduces signaling mediated by the receptor tyrosine kinase (RTK), but also regulates the stability of cytoskeletal network through a number of cytoskeleton-associated proteins, including Septin5, MAPs , and stathmin family members. KIDINS220 exists some isoforms with MV 194 kDa, 185 kDa, 115 kDa, and 60 kDa.

实验方案

Product Specific Protocols
WB protocol for KIDINS220 antibody 21856-1-APDownload protocol
IP protocol for KIDINS220 antibody 21856-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cell Biol

A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport.

Authors - Steinberg Florian F
humanWB

J Exp Med

Kidins220/ARMS binds to the B cell antigen receptor and regulates B cell development and activation.

Authors - Gina J Fiala
  • KD Validated
mouseWB

Sci Adv

Kidins220 and Aiolos promote thymic iNKT cell development by reducing TCR signals

Authors - Laurenz A Herr
humanWB

Nat Commun

Regulation of the endosomal SNX27-retromer by OTULIN.

Authors - Aurelia Stangl
humanWB,IP

Proc Natl Acad Sci U S A

A unique PDZ domain and arrestin-like fold interaction reveals mechanistic details of endocytic recycling by SNX27-retromer.

Authors - Matthew Gallon
human

Front Immunol

Patients' IgLON5 autoantibodies interfere with IgLON5-protein interactions

Authors - Jon Landa
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