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验证数据展示

  • WB analysis of HEK-293 using 27886-1-AP

    WB analysis of HEK-293 using 27886-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 27886-1-AP (KIAA2013 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 27886-1-AP

    WB analysis using 27886-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27886-1-AP (KIAA2013 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 27886-1-AP

    IHC staining of human ovary cancer using 27886-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 27886-1-AP (KIAA2013 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KIAA2013 Polyclonal antibody

KIAA2013 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27886-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, U2OS cells, mouse kidney tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27886-1-AP targets KIAA2013 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 KIAA2013 fusion protein Ag27379 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA2013
别名
观测分子量 65 kDa
GenBank蛋白编号BC035033
基因名称 KIAA2013
Gene ID (NCBI) 90231
RRIDAB_2881004
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYS2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA2013, also known as Q8IYS2 or MGC33867, is a single-pass transmembrane protein encoded by the KIAA2013 gene in humans. KIAA2013 has been found to play a role in the Endocannabinoid system. This system is made up of cannabinoid receptors 1 and 2 (CB1 and CB2) as well as the various ligands and enzymes that interact. The protein KIAA2013 has been found to be expressed within CB2 expressing cells. Both cannabinoid receptors are labeled as class A G-Protein Coupled Receptors, and CB2 is highly expressed within the human spleen and leukocytes. CB2, and by extension KIAA2013, are therefore targets of interest for therapeutic studies looking into diseases such as inflammatory bowel disease and rheumatoid arthritis(PMID:31616248 34752341).

实验方案

Product Specific Protocols
WB protocol for KIAA2013 antibody 27886-1-APDownload protocol
IHC protocol for KIAA2013 antibody 27886-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols