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验证数据展示

  • WB analysis using 30140-1-AP

    WB analysis using 30140-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30140-1-AP (KIAA1797 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 30140-1-AP

    WB analysis of HeLa using 30140-1-AP

    WB result of KIAA1797 antibody (30140-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-KIAA1797 transfected HeLa cells.

  • IF Staining of HeLa using 30140-1-AP

    IF Staining of HeLa using 30140-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using KIAA1797 antibody (30140-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Featured Product
  • KD/KO Validated

KIAA1797 Polyclonal antibody

KIAA1797 Polyclonal Antibody for WB, ELISA
Cat No. 30140-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, ELISA

别名

FLJ20375, KIAA1797

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, HeLa cells, Neuro-2a cells, SH-SY5Y cells, U-251 cells, C6 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30140-1-AP targets KIAA1797 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原

CatNo: Ag31233

Product name: Recombinant human KIAA1797 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 230-502 aa of BC001246

Sequence: LLSEATGKIFDLLPNKIRRKDLELYISIAKCLLEMTDDDANRIAQVTKSNIEKAAFVKLYLVSQGRFPLVNLTDMLSVAVQHREKEVLAWMILHSLYQARIVSHANTGVLKRMEWLLELMGYIRNVAYQSTSFHNTALDEALDFFLLIFATAVVAWADHTAPLLLGLSASWLPWHQENGPAGPVPSFLGRSPMHRVTLQEVLTLLPNSMALLLQKEPWKEQTQKFIDWLFSIMESPKEALSAQSRDLLKATLLSLRVLPEFKKKAVWTRAYGW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA1797
别名 FLJ20375, KIAA1797
观测分子量160-170 kDa
GenBank蛋白编号BC001246
基因名称 KIAA1797
Gene ID (NCBI) 54914
RRIDAB_3086244
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5VW36
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA1797 is a novel component of the focal adhesion complex expressed in glial and neuronal cells with a tumour suppressor function in gliomas, which is also named 'focadhesin' (PMID: 22427331).

实验方案

Product Specific Protocols
IF protocol for KIAA1797 antibody 30140-1-APDownload protocol
WB protocol for KIAA1797 antibody 30140-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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