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验证数据展示

  • WB analysis using 30857-1-AP

    WB analysis using 30857-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30857-1-AP (KIAA1715 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse cerebellum using 30857-1-AP

    IHC staining of mouse cerebellum using 30857-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 30857-1-AP (KIAA1715 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

KIAA1715 Polyclonal antibody

KIAA1715 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30857-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, IHC, ELISA

别名

DKFZp686G1442, KIAA1715, LNP, Protein lunapark, Ul, ulnaless

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells, HEK-293 cells, K-562 cells
Positive IHC detected inmouse cerebellum tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30857-1-AP targets KIAA1715 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 Human, mouse
免疫原 KIAA1715 fusion protein Ag33996 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA1715
别名 DKFZp686G1442, KIAA1715, LNP, Protein lunapark, Ul, ulnaless
观测分子量53 kDa
GenBank蛋白编号BC105132
基因名称 KIAA1715
Gene ID (NCBI) 80856
RRIDAB_3086424
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9C0E8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA1715, also known as LNPK, is a member of the conserved Lunapark protein family. KIAA1715 is an endoplasmic reticulum (ER) membrane protein consisting of an N-terminal domain important for N-myristoylation and ER targeting, two transmembrane domains and a unique zinc finger domain. It has 4 isoforms produced by alternative splicing and acts mainly as a stabilizer of negative membrane curvature (PMID: 27619977).

实验方案

Product Specific Protocols
WB protocol for KIAA1715 antibody 30857-1-APDownload protocol
IHC protocol for KIAA1715 antibody 30857-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols