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NCKAP5L Polyclonal antibody

NCKAP5L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat No : 21392-1-AP

Print datasheet

Synonyms

Cep169, FP1193, KIAA1602, Nck associated protein 5 like, NCKAP5L

验证数据展示

  • WB analysis of HeLa using 21392-1-AP

    WB analysis of HeLa using 21392-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21392-1-AP (NCKAP5L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin using 21392-1-AP

    IHC staining of human skin using 21392-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 21392-1-AP (NCKAP5L antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human skin using 21392-1-AP

    IHC staining of human skin using 21392-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 21392-1-AP (NCKAP5L antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21392-1-AP targets NCKAP5L in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA
Tested Reactivity human
Immunogen NCKAP5L fusion protein Ag16050 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA1602
Synonyms Cep169, FP1193, KIAA1602, Nck associated protein 5 like, NCKAP5L
Calculated Molecular Weight 1334 aa, 139 kDa
Observed Molecular Weight 145-150 kDa
GenBank Accession NumberBC110599
Gene Symbol KIAA1602
Gene ID (NCBI) 57701
RRIDAB_10860259
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HCH0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

NCKAP5L, also named as KIAA1602, has 4 isoforms. The target band is about 139-145 kDa for phosphorylation.

实验方案

Product Specific Protocols
WB protocol for NCKAP5L antibody 21392-1-APDownload protocol
IHC protocol for NCKAP5L antibody 21392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols