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验证数据展示

  • WB analysis using 86541-2-RR (same clone as 86541-2-PBS)

    WB analysis using 86541-2-RR (same clone as 86541-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86541-2-RR (Shootin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86541-2-PBS in a different storage buffer formulation.

  • WB analysis using 86541-2-RR (same clone as 86541-2-PBS)

    WB analysis using 86541-2-RR (same clone as 86541-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86541-2-RR (Shootin 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86541-2-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 86541-2-RR (same clone as 86541-2-PBS)

    IF Staining of SH-SY5Y using 86541-2-RR (same clone as 86541-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed SH-SY5Y cells using Shootin 1 antibody (86541-2-RR, Clone: 251435E5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86541-2-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP02499-1

    Cytometric bead array sample test of MP02499-1

    The mean Shootin 1 concentration was determined to be 50.1 ng/mL in SH-SY5Y cell extract based on a 1.4 mg/mL extract load, 47.7 ng/mL in Neuro-2a cell extract based on a 1.6 mg/mL extract load.

  • Cytometric bead array standard curve of MP02499-1

    Cytometric bead array standard curve of MP02499-1

    Cytometric bead array standard curve of MP02499-1, Shootin 1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 86541-2-PBS. Detection antibody: 86541-1-PBS. Standard: Ag18294. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 86541-2-RR

    Affinity and Kinetic Characterization of 86541-2-RR

    Biolayer interferometry (BLl) kinetic assays of 86541-2-RR against Human Shootin 1 were performed. The affinity constant is 0.965 nM.

Shootin 1 Recombinant monoclonal antibody, PBS Only (Capture)

Shootin 1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 86541-2-PBS

产品说明书

CloneNo. 251435E5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

SHTN1, KIAA1598, Shootin-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86541-2-PBS targets Shootin 1 as part of a matched antibody pair:

MP02499-1: 86541-2-PBS capture and 86541-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag18294

Product name: Recombinant human KIAA1598 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 834-1066 aa of BC022348

Sequence: TEEVTDLKRQAVEEMMDRIKKGVHLRPVNQTARPKTKPESSKGCESAVDELKGILGTLNKSTSSRSLKSLDPENSETELERILRRRKVTAEADSSSPTGILATSESKSMPVLGSVSSVTKTALNKKTLEAEFNSPSPPTPEPGEGPRKLEGCTSSKVTFQPPSSIGCRKKYIDGEKQAEPVVVLDPVSTHEPQTKDQVAEKDPTQHKEDEGEIQPENKEDSIENVRETDSSNC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 KIAA1598
别名 SHTN1, KIAA1598, Shootin-1
计算分子量 72 kDa
观测分子量 60-80 kDa
GenBank蛋白编号BC022348
基因名称 Shootin 1
Gene ID (NCBI) 57698
RRIDAB_3744954
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDA0MZ66
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Shootin 1, also known as SHTN1, KIAA1598, contains 3 coiled-coil domains and a proline-rich region. Shootin 1 is involved in generating internal asymmetric signals required for neuronal during stages 2 and 3. Shootin 1 plays a role in cytoskeletal organization by regulating the subcellular localization of phosphoinositide 3-kinase (PI3K) activity at the axonal growth cone. SHTN1 produces 2 splice variants, a short variant, SHTN1S, that lacks exons 15 and 16, and a long variant, SHTN1L, that includes exons 1 through 17(PMID: 17030985, 30733148).

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