验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83900-7-RR targets KIAA1586 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag36038 Product name: Recombinant human KIAA1586 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 426-749 aa of BC041125 Sequence: SLDDSISEIKQINHLKIFIDKIYSIYHQPNKNQTKLLGTVAKELETEIIKIGRVMGPRWAACSLQAATAVWHAYPILYMHFSHSYSGLAKRLANINFLQDLALMIDILEEFSVLSTALQSRSTNIKKAQKLIKRTIRALENLKIGTGKYESQIEDLIKSDKFKDIPFNKNNKFNALPRSILLDNIIQHMNLRLLSDRNHEDIFNYFDLLEPSTWPYEEITSPWIAGEKTLFHLCKILKYEVDLNDFREFVNNNIKSNNVSIPTTIYKAKKIVSTIAINSAEAERGFNLMNIICTRVRNSLTIDHVSDLMTINLLGKELADWDAT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | KIAA1586 |
| 别名 | 240972F10 |
| 计算分子量 | 787 aa, 90 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC041125 |
| 基因名称 | KIAA1586 |
| Gene ID (NCBI) | 57691 |
| RRID | AB_3671481 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | Q9HCI6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIAA1586, close to ZNF451, encodes a protein with similar N-terminal tandem SIMs, which has also shown elongase activity. ZNF451 is an E3 SUMO-protein ligase that facilitates UBE2I/UBC9-mediated SUMO2 modification of target proteins (PMID: 26524493, PMID: 35887358).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KIAA1586 antibody 83900-7-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
