验证数据展示
经过测试的应用
| Positive WB detected in | mouse pancreas tissue, rat pancreas |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32377-1-AP targets RIC1 in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag36426 Product name: Recombinant human KIAA1432 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 820-1054 aa of BC136616 Sequence: QTVVHCARKTEYALWNYLFAAVGNPKDLFEECLMAQDLDTAASYLIILQNMEVPAVSRQHATLLFNTALEQGKWDLCRHMIRFLKAIGSGESETPPSTPTAQEPSSSGGFEFFRNRSISLSQSAENVPASKFSLQKTLSMPSGPSGKRWSKDSDCAENMYIDMMLWRHARRLLEDVRLKDLGCFAAQLGFELISWLCKERTRAARVDNFVIALKRLHKDFLWPLPIIPASSISSP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KIAA1432 |
| 别名 | CIP150, KIAA1432, Protein RIC1 homolog, Q4ADV7 |
| 观测分子量 | 150 kDa |
| GenBank蛋白编号 | BC136616 |
| 基因名称 | KIAA1432 |
| Gene ID (NCBI) | 57589 |
| RRID | AB_3742587 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q4ADV7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RIC1 (RAB6A GEF complex partner 1) is a protein that plays a critical role in intracellular membrane trafficking. It encodes a protein of 1056 amino acid (aa) residues, including a putative nuclear localisation sequence (PMID: 9099877). Ric1 is composed of two β-propellers and a C-terminal α-solenoid domain that remodels the Rab6 nucleotide-binding pocket to facilitate nucleotide exchange (PMID: 39632878). RIC1 proteins play important roles in vesicular trafficking, regulation of membrane dynamics, cell division and signalling, and are essential for maintaining normal cellular function.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for RIC1 antibody 32377-1-AP | Download protocol |
| WB protocol for RIC1 antibody 32377-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
