验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, SH-SY5Y cells, SK-N-SH cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
32570-1-AP targets KIAA1211 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag38501 Product name: Recombinant human KIAA1211 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 430-580 aa of NM_020722.1 Sequence: LEDQERLKPEGQREHSEEPGICEEQNPEAERRREQQGRSGDFQGADRPGPEEKREEGDTEPLLKQEGPVEAAQPPVERKEAAALEQGRKVEELRWQEVDERQTMPRPYTFQVSSGGKQILFPKVNLSPVTPAKDTGLTAAPQEPKAPKASP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KIAA1211 |
| 别名 | Cancer-related regulator of actin dynamics, Capping protein-inhibiting regulator of actin dynamics, CRACD, CRAD |
| 计算分子量 | 137kDa，1233aa |
| 观测分子量 | 137 kDa |
| GenBank蛋白编号 | NM_020722.1 |
| 基因名称 | KIAA1211 |
| Gene ID (NCBI) | 57482 |
| RRID | AB_3742658 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q6ZU35 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
KIAA1211 locates at human chromosome 4q12, and has only one transcript (NM_020722.1, 4914 bp). KIAA1211 encoded a 137 kDa nuclear protein and highly expressed in the brain cerebral cortex. Furthermore, it is localized to the microtubules and the centrosomes and is subcellularly located in the nucleus. In recent years, it has been found that the KIAA1211 gene is closely associated with the development and prognosis of various cancers (PMID: 31777604).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for KIAA1211 antibody 32570-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
