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UFL1 Polyclonal antibody

UFL1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26087-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse and More (1)

应用

WB, IHC, ELISA and More (2)

KIAA0776, MAXER, EC:2.3.2.-, E3 UFM1-protein transferase 1, E3 UFM1-protein ligase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293T cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy UFL1 Ready-To-Use IHC Kit. UFL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26087-1-AP targets UFL1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse
文献引用反应性human, mouse, bovine
免疫原 UFL1 fusion protein Ag23404 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA0776
别名 KIAA0776, MAXER, EC:2.3.2.-, E3 UFM1-protein transferase 1, E3 UFM1-protein ligase 1
观测分子量80 kDa; 100 kDa
GenBank蛋白编号BC036379
基因名称 KIAA0776
Gene ID (NCBI) 23376
RRIDAB_2880370
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO94874
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for UFL1 antibody 26087-1-APDownload protocol
IHC protocol for UFL1 antibody 26087-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle

Mol Cell

An Epstein-Barr virus protein interaction map reveals NLRP3 inflammasome evasion via MAVS UFMylation

Authors - Stephanie Pei Tung Yiu
mouseWB,IF

Oxid Med Cell Longev

UFM1-Specific Ligase 1 Ligating Enzyme 1 Mediates Milk Protein and Fat Synthesis-Related Gene Expression via the JNK Signaling Pathway in Mouse Mammary Epithelial Cells.

Authors - Meiqian Kuang
  • KO Validated
bovineWB,IHC,IF

Cell Stress Chaperones

Protective effects of UFL1 against endoplasmic reticulum stress-induced autophagy in bovine mammary epithelial cells.

Authors - Meiqian Kuang
  • KD Validated
bovineWB

In Vitro Cell Dev Biol Anim

UFL1 regulates milk protein and fat synthesis-related gene expression of bovine mammary epithelial cells probably via the mTOR signaling pathway.

Authors - Chengmin Li
  • KD Validated
bovineIF,IHC

Biomolecules

UFL1 Alleviates LPS-Induced Apoptosis by Regulating the NF-κB Signaling Pathway in Bovine Ovarian Granulosa Cells.

Authors - Xinling Wang
bovineWB,IF,IHC

Oxid Med Cell Longev

UFL1 Alleviates Lipopolysaccharide-Induced Cell Damage and Inflammation via Regulation of the TLR4/NF-κB Pathway in Bovine Mammary Epithelial Cells.

Authors - Chengmin Li
  • KD Validated
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