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验证数据展示

  • WB analysis of mouse brain using 24921-1-AP

    WB analysis of mouse brain using 24921-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24921-1-AP (KIAA0774 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 24921-1-AP

    IHC staining of human heart using 24921-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24921-1-AP (KIAA0774 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human heart using 24921-1-AP

    IHC staining of human heart using 24921-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 24921-1-AP (KIAA0774 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HepG2 using 24921-1-AP

    IF Staining of HepG2 using 24921-1-AP

    Immunofluorescent analysis of HepG2 cells using 24921-1-AP (KIAA0774 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

KIAA0774 Polyclonal antibody

KIAA0774 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 24921-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

别名

TIP150, MTUS2, Microtubule-associated tumor suppressor candidate 2, Microtubule plus-end tracking protein TIP150, CAZIP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, mouse heart tissue
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

24921-1-AP targets KIAA0774 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 KIAA0774 fusion protein Ag20593 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA0774
别名 TIP150, MTUS2, Microtubule-associated tumor suppressor candidate 2, Microtubule plus-end tracking protein TIP150, CAZIP
计算分子量 1369 aa, 150 kDa
观测分子量 150 kDa, 41 kDa
GenBank蛋白编号BC032481
基因名称 KIAA0774
Gene ID (NCBI) 23281
RRIDAB_2879797
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5JR59
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

KIAA0774, also named as MTUS2, CAZIP and TIP150, binds microtubules. It may regulate the dynamics of microtubules at their growing distal tip. KIAA0774 has three isoforms with MW 150 kDa, 41 kDa and 30 kDa.

实验方案

Product Specific Protocols
WB protocol for KIAA0774 antibody 24921-1-APDownload protocol
IHC protocol for KIAA0774 antibody 24921-1-APDownload protocol
IF protocol for KIAA0774 antibody 24921-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols