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KIAA0528 Polyclonal antibody

KIAA0528 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Cat No : 21138-1-AP

Print datasheet

Synonyms

C2CD5, CDP138, KIAA0528

验证数据展示

  • WB analysis using 21138-1-AP

    WB analysis using 21138-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 21138-1-AP

    IHC staining of mouse small intestine using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse small intestine using 21138-1-AP

    IHC staining of mouse small intestine using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 21138-1-AP

    IF Staining of HepG2 using 21138-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIAA0528 antibody 21138-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of HeLa using 21138-1-AP

    IP experiment of HeLa using 21138-1-AP

    IP result of anti-KIAA0528 (IP:21138-1-AP, 4ug; Detection:21138-1-AP 1:700) with HeLa cells lysate 520ug.

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21138-1-AP targets KIAA0528 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, IP, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen KIAA0528 fusion protein Ag15590 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name KIAA0528
Synonyms C2CD5, CDP138, KIAA0528
Calculated Molecular Weight 1000 aa, 110 kDa
Observed Molecular Weight 115 kDa
GenBank Accession NumberBC042498
Gene Symbol KIAA0528
Gene ID (NCBI) 9847
RRIDAB_10858480
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86YS7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIAA0528 antibody 21138-1-APDownload protocol
IHC protocol for KIAA0528 antibody 21138-1-APDownload protocol
IF protocol for KIAA0528 antibody 21138-1-APDownload protocol
IP protocol for KIAA0528 antibody 21138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols