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验证数据展示

  • WB analysis using 21138-1-AP

    WB analysis using 21138-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21138-1-AP (KIAA0528 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse small intestine using 21138-1-AP

    IHC staining of mouse small intestine using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C2CD5 antibody included
  • IHC staining of mouse small intestine using 21138-1-AP

    IHC staining of mouse small intestine using 21138-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 21138-1-AP (KIAA0528 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with C2CD5 antibody included
  • IF Staining of HepG2 using 21138-1-AP

    IF Staining of HepG2 using 21138-1-AP

    Immunofluorescent analysis of HepG2 cells, using KIAA0528 antibody 21138-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IP experiment of HeLa using 21138-1-AP

    IP experiment of HeLa using 21138-1-AP

    IP result of anti-KIAA0528 (IP:21138-1-AP, 4ug; Detection:21138-1-AP 1:700) with HeLa cells lysate 520ug.

KIAA0528 Polyclonal antibody

KIAA0528 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21138-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

CDP138, C2CD5, C2 domain-containing protein 5, C2 domain-containing phosphoprotein of 138 kDa

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, rat brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inmouse small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy C2CD5 Ready-To-Use IHC Kit. C2CD5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21138-1-AP targets KIAA0528 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 KIAA0528 fusion protein Ag15590 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 KIAA0528
别名 CDP138, C2CD5, C2 domain-containing protein 5, C2 domain-containing phosphoprotein of 138 kDa
计算分子量 1000 aa, 110 kDa
观测分子量 110-125 kDa
GenBank蛋白编号BC042498
基因名称 KIAA0528
Gene ID (NCBI) 9847
RRIDAB_10858480
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86YS7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for KIAA0528 antibody 21138-1-APDownload protocol
IHC protocol for KIAA0528 antibody 21138-1-APDownload protocol
IF protocol for KIAA0528 antibody 21138-1-APDownload protocol
IP protocol for KIAA0528 antibody 21138-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols