验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, Jurkat cells, MG-63 cells, U2OS cells |
| Positive IP detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
34075-1-AP targets AP5Z1/SPG48 in WB, IP, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IP, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag26954 Product name: Recombinant human KIAA0415 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 260-364 aa of BC037399 Sequence: TLDTDDRSEQEGSTLSVISATSSAGRLLPPRERLREVAFEYCQRLIEQSNRRALRKGDSDLQKACLVEAVLVLDVLCRQDPSFLYRSLSCLKALHGRVRGDPASV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KIAA0415 |
| 别名 | KIAA0415, Adaptor-related protein complex 5 zeta subunit, AP-5 complex subunit zeta-1, AP5Z1, SPG48 |
| 计算分子量 | 807 aa, 88 kDa |
| 观测分子量 | 88 kDa |
| GenBank蛋白编号 | BC037399 |
| 基因名称 | AP5Z1/SPG48 |
| Gene ID (NCBI) | 9907 |
| RRID | AB_3743150 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | O43299 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AP5Z1, also known as SPG48 and KIAA0415, is a part of AP-5. Biallelic mutations in the AP5Z1 gene encoding the AP5Z1 subunit have been described in a small number of patients with hereditary spastic paraplegia (HSP). AP5Z1 physically interacts with other HSP proteins and that patient cells are sensitive to DNA damaging drugs.
实验方案
| Product Specific Protocols | |
|---|---|
| IP protocol for AP5Z1/SPG48 antibody 34075-1-AP | Download protocol |
| WB protocol for AP5Z1/SPG48 antibody 34075-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
