验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, L02 cells, mouse brain tissue, mouse liver tissue |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
产品信息
20959-1-AP targets MRPP3 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag15196 Product name: Recombinant human KIAA0391 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 230-583 aa of BC032221 Sequence: LLLLEDIKKVITPSKKNYNDCIQGALLHQDVNTAWNLYQELLGHDIVPMLETLKAFFDFGKDIKDDNYSNKLLDILSYLRNNQLYPGESFAHSIKTWFESVPGKQWKGQFTTVRKSGQCSGCGKTIESIQLSPEEYECLKGKIMRDVIDGGDQYRKTTPQELKRFENFIKSRPPFDVVIDGLNVAKMFPKVRESQLLLNVVSQLAKRNLRLLVLGRKHMLRRSSQWSRDEMEEVQKQASCFFADDISEDDPFLLYATLHSGNHCRFITRDLMRDHKACLPDAKTQRLFFKWQQGHQLAIVNRFPGSKLTFQRILSYDTVVQTTGDSWHIPYDEDLVERCSCEVPTKWLCLHQKT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KIAA0391 |
| 别名 | KIAA0391, EC:3.1.26.5, Mitochondrial ribonuclease P catalytic subunit, PRORP, Protein only RNase P catalytic subunit |
| 计算分子量 | 583 aa, 67 kDa |
| 观测分子量 | 65-67 kDa, 56 kDa |
| GenBank蛋白编号 | BC032221 |
| 基因名称 | MRPP3 |
| Gene ID (NCBI) | 9692 |
| RRID | AB_10860109 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O15091 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
MRPP3 (Mitochondrial ribonuclease P protein 3), originally known as KIAA0391, has been identified as a member of human mitochondrial RNase P (mtRNase P), the endonuclease responsible for tRNA 5' end maturation by removing extra nucleotides from tRNA precursors at their 5' termini. Recently Daniel et al. reported that Rnase P and other mitochondrial RNA processing enzymes are localized near mt DNA nucleoids where they can initiate process of primary transcripts. (24703694). Western detected isoforms of MRPP3 as 67 kDa, 56 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for MRPP3 antibody 20959-1-AP | Download protocol |
| WB protocol for MRPP3 antibody 20959-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Initial steps in RNA processing and ribosome assembly occur at mitochondrial DNA nucleoids. | ||
J Biol Chem RNase P generated tRF(Ser-GCT) promotes fat storage in adipocytes via Adrb2 signaling.
| ||
EMBO Mol Med Hyperglycemia impairs cognitive function by inducing mitochondrial damage through lactylation of LRPPRC at K223. |
