验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, MCF-7 cells, MG-63 cells, U2OS cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20537-1-AP targets PAF15 in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 |
CatNo: Ag14522 Product name: Recombinant human KIAA0101 protein Source: e coli.-derived, PKG Tag: GST Domain: 1-111 aa of BC005832 Sequence: MVRTKADSVPGTYRKVVAARAPRKVLGSSTSATNSTSVSSRKAENKYAGGNPVCVRPTPKWQKGIGEFFRLSPKDSEKENQIPEEAGSSGLGKAKRKACPLQPDHTNDEKE 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | KIAA0101 |
| 别名 | KIAA0101, HCV NS5A-transactivated protein 9, Hepatitis C virus NS5A-transactivated protein 9, L5, NS5ATP9 |
| 计算分子量 | 111 aa, 12 kDa |
| 观测分子量 | 15-17 kDa |
| GenBank蛋白编号 | BC005832 |
| 基因名称 | KIAA0101 |
| Gene ID (NCBI) | 9768 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15004 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
PAF15, also known as PCLAF/KIAA0101, located at 15q14, is a small nuclear protein that binds to PCNA. Its expression fluctuated with the cell cycle, with high expression in S/G2 phase and degradation in G1 phase. Anchoring replication fork by PIP motif, specifically stabilizing the synthesis of lag chain, and regulating S-phase checkpoint; Participate in cross-injury repair when DNA is damaged. The gene is abnormally overexpressed in various solid tumors, which drives tumor progress by disturbing genome stability, promoting proliferation and antagonizing apoptosis. The overexpression indicates poor prognosis, and it is a tumor prognosis marker and potential therapeutic target. There are reports of 15kDa bands and 25 kDa bands with ubiquitination modification (PMID: 41606318).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PAF15 antibody 20537-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
